Biomaterial Database

Characterization of excitatory and inhibitory motor neurons to the guinea pig lower esophageal sphincter. 1996

S J Brookes, and B N Chen, and W M Hodgson, and M Costa

Department of Human Physiology and Centre for Neuroscience, Flinders University, Bedford Park, South Australia, Australia.

OBJECTIVE The lower esophageal sphincter is innervated primarily by enteric motor neurons. The somata of excitatory and inhibitory motor neurons were identified and mapped. METHODS Retrograde labeling in organotypic culture and immunohistochemistry were used to identify motor neuron somata. RESULTS 1,1'-Didodecyl-3,3,3',3'-tetramethyl indocarbocyanine perchlorate (Dil) on the left side of the sphincter labeled descending motor neurons located up to 26 mm along the esophagus and locally (within 2 mm) and gastric motor neurons. Dil applied to the right side of the sphincter labeled descending and local motor neurons but very few gastric motor neurons. Inhibitory motor neuron cell bodies, identified by nitric oxide synthase immunoreactivity, accounted for 86% +/- 4% (n = 6) of descending motor neurons and 53% +/- 4% of local motor neurons labeled from the right side. Excitatory motor neurons, immunoreactive for choline acetyltransferase, accounted for 20% +/- 3% (n = 6) of descending motor neurons and for 47% +/- 4% of local motor neurons. All motor neurons were unipolar, but inhibitory motor neurons were significantly larger than excitatory neurons. CONCLUSIONS The lower esophageal sphincter is innervated by local excitatory and inhibitory motor neurons and by descending esophageal inhibitory neurons. The oblique muscle, supplied by gastric motor neurons, is closely associated with the gastroesophageal junction.

UI	MeSH Term	Description	Entries
D007150	Immunohistochemistry	Histochemical localization of immunoreactive substances using labeled antibodies as reagents.	Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008297	Male		Males
D009046	Motor Neurons	Neurons which activate MUSCLE CELLS.	Neurons, Motor,Alpha Motorneurons,Motoneurons,Motor Neurons, Alpha,Neurons, Alpha Motor,Alpha Motor Neuron,Alpha Motor Neurons,Alpha Motorneuron,Motoneuron,Motor Neuron,Motor Neuron, Alpha,Motorneuron, Alpha,Motorneurons, Alpha,Neuron, Alpha Motor,Neuron, Motor
D009130	Muscle, Smooth	Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed)	Muscle, Involuntary,Smooth Muscle,Involuntary Muscle,Involuntary Muscles,Muscles, Involuntary,Muscles, Smooth,Smooth Muscles
D002795	Choline O-Acetyltransferase	An enzyme that catalyzes the formation of acetylcholine from acetyl-CoA and choline. EC 2.3.1.6.	Choline Acetylase,Choline Acetyltransferase,Acetylase, Choline,Acetyltransferase, Choline,Choline O Acetyltransferase,O-Acetyltransferase, Choline
D004943	Esophagogastric Junction	The area covering the terminal portion of ESOPHAGUS and the beginning of STOMACH at the cardiac orifice.	Gastroesophageal Junction,Gastroesophageal Junctions,Junction, Esophagogastric,Junction, Gastroesophageal,Junctions, Gastroesophageal
D005260	Female		Females
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013270	Stomach	An organ of digestion situated in the left upper quadrant of the abdomen between the termination of the ESOPHAGUS and the beginning of the DUODENUM.	Stomachs