-transparent.png){kind=logo}
BACKGROUND Satellite cell necrosis is a histopathologic hallmark of cutaneous graft-versus-host disease (GVHD) after allogeneic bone marrow transplantation. Although this interaction of lymphocytes with keratinocytes has features of immune cytolytic destruction, the details of the process are not known. OBJECTIVE Our purpose was to investigate whether apoptosis is involved in the process of GVHD. METHODS We used TdT-mediated dUTP-biotin nick end labeling (TUNEL) technique with biotinylated nucleotides. Formalin-fixed, paraffin-embedded sections from skin biopsy specimens of patients with GVHD and normal skin were studied. RESULTS Labeling of scattered individual keratinocytes in the epidermis and adnexal epithelium undergoing cytolytic degeneration and a proportion of the adjacent lymphocytes was noted in all specimens of GVHD. CONCLUSIONS The positive staining of keratinocytes by the TUNEL method indicates that cell destruction in cutaneous GVHD involves apoptosis.
R G Langley, and
N Walsh, and
T Nevill, and
L Thomas, and
G Rowden
April 1995,
Presse medicale (Paris, France : 1983),
R G Langley, and
N Walsh, and
T Nevill, and
L Thomas, and
G Rowden
May 2003,
Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete,
R G Langley, and
N Walsh, and
T Nevill, and
L Thomas, and
G Rowden
August 1987,
British medical journal (Clinical research ed.),
R G Langley, and
N Walsh, and
T Nevill, and
L Thomas, and
G Rowden
May 1998,
Archives of dermatology,
R G Langley, and
N Walsh, and
T Nevill, and
L Thomas, and
G Rowden
February 2018,
Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete,
R G Langley, and
N Walsh, and
T Nevill, and
L Thomas, and
G Rowden
October 1999,
The British journal of dermatology,
R G Langley, and
N Walsh, and
T Nevill, and
L Thomas, and
G Rowden
April 2012,
Journal of the American Academy of Dermatology,
R G Langley, and
N Walsh, and
T Nevill, and
L Thomas, and
G Rowden
November 2020,
Dermatology online journal,
R G Langley, and
N Walsh, and
T Nevill, and
L Thomas, and
G Rowden
July 1985,
The Journal of investigative dermatology,
R G Langley, and
N Walsh, and
T Nevill, and
L Thomas, and
G Rowden
August 1987,
The American Journal of dermatopathology,
