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Efficacy of passive transfer of proteins from preparative polyacrylamide gels to nitrocellulose membranes. 1995

S Khurana, and M K
Department of Zoology, University of Delhi, India.

Passive transfer of proteins from preparative polyacrylamide gels to nitrocellulose membrane (NTC) was investigated. The transfer was carried out without the removal of sodium dodecyl sulphate from the denaturing gels. The transfer was quick, easy to perform, and does not require any apparatus. More than one replica of the same band can be obtained by successive transfer to more than one NTC without significantly affecting the Coomassie Brilliant Blue (CBB) staining ability of protein bands left in the gel. A fraction of protein transferred passively in the absence of electrical current from as low as 1.5 micrograms of protein per band in a 2 mm thick gel was found sufficient for detection with amido black staining, and more so by immunostaining. The method was found highly satisfactory for seven proteins (14 kDa to 66 kDa) checked in the present study and can be adopted, after checking its efficacy for any protein, for routine qualitative tests.

UI MeSH Term Description Entries
D008567 Membranes, Artificial Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION. Artificial Membranes,Artificial Membrane,Membrane, Artificial
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D011506 Proteins Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein. Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D003101 Collodion A nitrocellulose solution in ether and alcohol. Collodion has a wide range of uses in industry including applications in the manufacture of photographic film, in fibers, in lacquers, and in engraving and lithography. In medicine it is used as a drug solvent and a wound sealant. Nitrocellulose,Celloidin,Cellulose Nitrate,Collodion Cotton,Pyroxylin,Cotton, Collodion,Nitrate, Cellulose
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs

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