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Quantification of provirus copies is important in the context of different biological questions. The most reliable approach for DNA quantification is a PCR based on coamplification of two templates of similar length, the target sequence and the reference template, sharing the same primer recognition sequences. During the amplification, the two templates compete for the same primer set (competitive PCR, or cPCR) AND consequently amplify at the same rate independently of the number of cycles. The amplified products can be distingushed by their different lengths. After densitometrical analysis, the proviral copy number of experimentally feline immunodeficiency virus infected cats could be calculated, since a known amount of reference template was used. The method described here proved to be very sensitive (10 copies for the competitor-DNA) and was used to quantitate the proviral load during several experiments in which the influence of periodical immunestimulations and the effect of vaccines on the virus load was studied.
K Allenspach, and
M Amacker, and
C M Leutenegger, and
M Hottiger, and
R Hofmann-Lehmann, and
U Hübscher, and
M Pistello, and
H Lutz
August 1998,
Journal of clinical microbiology,
K Allenspach, and
M Amacker, and
C M Leutenegger, and
M Hottiger, and
R Hofmann-Lehmann, and
U Hübscher, and
M Pistello, and
H Lutz
July 1997,
Journal of virological methods,
K Allenspach, and
M Amacker, and
C M Leutenegger, and
M Hottiger, and
R Hofmann-Lehmann, and
U Hübscher, and
M Pistello, and
H Lutz
December 1997,
Journal of virological methods,
K Allenspach, and
M Amacker, and
C M Leutenegger, and
M Hottiger, and
R Hofmann-Lehmann, and
U Hübscher, and
M Pistello, and
H Lutz
December 2001,
BioTechniques,
K Allenspach, and
M Amacker, and
C M Leutenegger, and
M Hottiger, and
R Hofmann-Lehmann, and
U Hübscher, and
M Pistello, and
H Lutz
February 2006,
Molecular and cellular probes,
K Allenspach, and
M Amacker, and
C M Leutenegger, and
M Hottiger, and
R Hofmann-Lehmann, and
U Hübscher, and
M Pistello, and
H Lutz
June 1995,
The Journal of veterinary medical science,
K Allenspach, and
M Amacker, and
C M Leutenegger, and
M Hottiger, and
R Hofmann-Lehmann, and
U Hübscher, and
M Pistello, and
H Lutz
February 2001,
Meat science,
K Allenspach, and
M Amacker, and
C M Leutenegger, and
M Hottiger, and
R Hofmann-Lehmann, and
U Hübscher, and
M Pistello, and
H Lutz
December 1997,
Journal of virological methods,
K Allenspach, and
M Amacker, and
C M Leutenegger, and
M Hottiger, and
R Hofmann-Lehmann, and
U Hübscher, and
M Pistello, and
H Lutz
May 2023,
Journal of virological methods,
K Allenspach, and
M Amacker, and
C M Leutenegger, and
M Hottiger, and
R Hofmann-Lehmann, and
U Hübscher, and
M Pistello, and
H Lutz
July 1994,
BioTechniques,
