Biomaterial Database

A study of the bone morphogenetic protein derived from bovine demineralized dentin matrix. 1996

H Mizutani, and K Mera, and M Ueda, and H Iwata

Department of Oral Surgery, Nagoya University School of Medicine, Japan.

We tried to extract and purify bone morphogenetic protein (BMP) from bovine demineralized dentin matrix (DDM). Crude dentin BMP (d-BMP) was extracted from DDM in 3 sequential steps. Purification of crude d-BMP was carried out by liquid chromatography. The molecular weight and the isoelectric point of the purified d-BMP were determined by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and electrofocusing. All fractioned samples were bioassayed in the thigh muscle pouches of AKR strain mice to test their ability to induce new bone formation. A sequence of 3 extraction steps worked effectively to obtain crude d-BMP. The purified d-BMP was shown to be homogeneous on high performance liquid chromatography (HPLC) and SDS-PAGE. The molecular weight and the pI were 25 kDa and 6.5, respectively. The amino acid composition was different from that of known bone-derived BMP. The purified d-BMP induced new bone formation in the thigh muscle pouches. The molecular weight, pI and amino acid composition were different from those of bone-derived BMP.

UI	MeSH Term	Description	Entries
D008903	Minerals	Native, inorganic or fossilized organic substances having a definite chemical composition and formed by inorganic reactions. They may occur as individual crystals or may be disseminated in some other mineral or rock. (Grant & Hackh's Chemical Dictionary, 5th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Mineral
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D003804	Dentin	The hard portion of the tooth surrounding the pulp, covered by enamel on the crown and cementum on the root, which is harder and denser than bone but softer than enamel, and is thus readily abraded when left unprotected. (From Jablonski, Dictionary of Dentistry, 1992)	Dentine,Dentines,Dentins
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D019485	Bone Morphogenetic Proteins	Bone-growth regulatory factors that are members of the transforming growth factor-beta superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.	Bone Morphogenetic Protein,Morphogenetic Protein, Bone,Morphogenetic Proteins, Bone