-transparent.png){kind=logo}
Human cord blood (HuCB) can colonize a murine fetal thymus organ culture (FTOC) and generate phenotypically immature (CD4+ CD8+) and mature (CD4+ CD8-; CD4- CD8+) T cells. We have used this model system to demonstrate that the human T cells that develop in this culture system can be infected with HIV-1. A cytopathic and non-cytopathic patient isolate of HIV-1 were used to infect FTOC established using C.B-17 or NOD/LtSz.scid/scid strain fetal thymic lobes colonized with HuCB. At 13-15 days after infection, FTOC were placed in co-culture with human PHA-blasts. These co-cultures demonstrated the presence of replicating HIV-1. Few human CD45+ cells were detectable in the thymic lobes that were infected with HIV-1, while high numbers of human CD45+ T cells were present in the uninfected cultures. These results demonstrate the cytopathicity of HIV-1 on human T lymphocytes that have developed in a HuCB colonized FTOC system.
D L Greiner, and
L D Shultz, and
D Deluca, and
J H Leif, and
S W Christianson, and
R M Hesselton
April 1993,
European journal of immunology,
D L Greiner, and
L D Shultz, and
D Deluca, and
J H Leif, and
S W Christianson, and
R M Hesselton
January 1994,
Advances in experimental medicine and biology,
D L Greiner, and
L D Shultz, and
D Deluca, and
J H Leif, and
S W Christianson, and
R M Hesselton
May 2000,
Blood,
D L Greiner, and
L D Shultz, and
D Deluca, and
J H Leif, and
S W Christianson, and
R M Hesselton
February 2000,
Journal of immunology (Baltimore, Md. : 1950),
D L Greiner, and
L D Shultz, and
D Deluca, and
J H Leif, and
S W Christianson, and
R M Hesselton
January 1999,
In vitro cellular & developmental biology. Animal,
D L Greiner, and
L D Shultz, and
D Deluca, and
J H Leif, and
S W Christianson, and
R M Hesselton
January 2005,
Methods in molecular medicine,
D L Greiner, and
L D Shultz, and
D Deluca, and
J H Leif, and
S W Christianson, and
R M Hesselton
July 1994,
The Journal of experimental medicine,
D L Greiner, and
L D Shultz, and
D Deluca, and
J H Leif, and
S W Christianson, and
R M Hesselton
January 2023,
Methods in molecular biology (Clifton, N.J.),
D L Greiner, and
L D Shultz, and
D Deluca, and
J H Leif, and
S W Christianson, and
R M Hesselton
January 2022,
Methods in molecular biology (Clifton, N.J.),
D L Greiner, and
L D Shultz, and
D Deluca, and
J H Leif, and
S W Christianson, and
R M Hesselton
October 1998,
Journal of immunology (Baltimore, Md. : 1950),
