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Vertebrate chromosomes terminate in variable numbers of T2AG3 nucleotide repeats. In order to study telomere repeats at individual chromosomes, we developed novel, quantitative fluorescence in situ hybridization procedures using labeled (C3TA2)3 peptide nucleic acid and digital imaging microscopy. Telomere fluorescence intensity values from metaphase chromosomes of cultured human hematopoietic cells decreased with the replication history of the cells, varied up to six-fold within a metaphase, and were similar between sister chromatid telomeres. Surprisingly, telomere fluorescence intensity values within normal adult bone marrow metaphases did not show a normal distribution, suggesting that a minimum number of repeats at each telomere is required and/or maintained during normal hematopoiesis.
P M Lansdorp, and
N P Verwoerd, and
F M van de Rijke, and
V Dragowska, and
M T Little, and
R W Dirks, and
A K Raap, and
H J Tanke
May 2021,
International journal of molecular sciences,
P M Lansdorp, and
N P Verwoerd, and
F M van de Rijke, and
V Dragowska, and
M T Little, and
R W Dirks, and
A K Raap, and
H J Tanke
November 2017,
Placenta,
P M Lansdorp, and
N P Verwoerd, and
F M van de Rijke, and
V Dragowska, and
M T Little, and
R W Dirks, and
A K Raap, and
H J Tanke
August 2004,
Cancer letters,
P M Lansdorp, and
N P Verwoerd, and
F M van de Rijke, and
V Dragowska, and
M T Little, and
R W Dirks, and
A K Raap, and
H J Tanke
January 1988,
Proceedings of the National Academy of Sciences of the United States of America,
P M Lansdorp, and
N P Verwoerd, and
F M van de Rijke, and
V Dragowska, and
M T Little, and
R W Dirks, and
A K Raap, and
H J Tanke
August 2001,
Nucleic acids research,
P M Lansdorp, and
N P Verwoerd, and
F M van de Rijke, and
V Dragowska, and
M T Little, and
R W Dirks, and
A K Raap, and
H J Tanke
April 2016,
Bulletin of experimental biology and medicine,
P M Lansdorp, and
N P Verwoerd, and
F M van de Rijke, and
V Dragowska, and
M T Little, and
R W Dirks, and
A K Raap, and
H J Tanke
November 2015,
Journal of assisted reproduction and genetics,
P M Lansdorp, and
N P Verwoerd, and
F M van de Rijke, and
V Dragowska, and
M T Little, and
R W Dirks, and
A K Raap, and
H J Tanke
May 2014,
Reproductive biomedicine online,
P M Lansdorp, and
N P Verwoerd, and
F M van de Rijke, and
V Dragowska, and
M T Little, and
R W Dirks, and
A K Raap, and
H J Tanke
January 2001,
Methods in cell biology,
P M Lansdorp, and
N P Verwoerd, and
F M van de Rijke, and
V Dragowska, and
M T Little, and
R W Dirks, and
A K Raap, and
H J Tanke
January 2002,
Methods in molecular medicine,
