[Evaluation of ELISA and counterimmunoelectrophoresis in diagnosis of human neurocysticercosis in Chile]. 1995

C Retamal, and I Zulantay, and H Sariego, and R Melo, and W Apt
Unidad de Parasitología, Universidad de Chile, Santiago.

The aim of this work was to assess the diagnostic accuracy for neurocysticercosis, of ELISA and counterimmunoelectrophoresis techniques, in sera and cerebrospinal fluid. Two hundred eight serum samples (47 coming from patients with confirmed cysticercosis) and 87 cerebrospinal fluid samples (27 coming from patients with confirmed cysticercosis) were analyzed. A crude and standardized extract of swine muscle cysticercus cellulose was used as antigen. ELISA and counter immunoelectrophoresis had a 100% specificity in cerebrospinal fluid. In sera, counterimmunoelectrophoresis had a 94.1% specificity. In sera and cerebrospinal fluid, ELISA had a 85.1% sensitivity. Cross reactions were observed in sera of patients with confirmed hydatidosis. Thus, the high specificity of both techniques in cerebrospinal fluid is probably due to the low incidence of cerebral hydatidosis in Chile. It is concluded that for the diagnosis of neurocysticercosis, antibodies against cysticercus cellulosae must be sought paralelly in serum and cerebrospinal fluid using ELISA and counterimmunoelectrophoresis.

UI MeSH Term Description Entries
D008297 Male Males
D001927 Brain Diseases Pathologic conditions affecting the BRAIN, which is composed of the intracranial components of the CENTRAL NERVOUS SYSTEM. This includes (but is not limited to) the CEREBRAL CORTEX; intracranial white matter; BASAL GANGLIA; THALAMUS; HYPOTHALAMUS; BRAIN STEM; and CEREBELLUM. Intracranial Central Nervous System Disorders,Brain Disorders,CNS Disorders, Intracranial,Central Nervous System Disorders, Intracranial,Central Nervous System Intracranial Disorders,Encephalon Diseases,Encephalopathy,Intracranial CNS Disorders,Brain Disease,Brain Disorder,CNS Disorder, Intracranial,Encephalon Disease,Encephalopathies,Intracranial CNS Disorder
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D002677 Chile A country in southern South America, bordering the South Pacific Ocean, between Argentina and Peru.
D003378 Counterimmunoelectrophoresis Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow. Electrosyneresis,Immunoelectroosmophoresis,Immunoelectrophoresis, Countercurrent,Immunoelectrophoresis, Crossover,Countercurrent Immunoelectrophoresis,Crossover Immunoelectrophoresis
D003551 Cysticercosis Infection with CYSTICERCUS, the larval form of the various tapeworms of the genus Taenia (usually T. solium in man). In humans they penetrate the intestinal wall and invade subcutaneous tissue, brain, eye, muscle, heart, liver, lung, and peritoneum. Brain involvement results in NEUROCYSTICERCOSIS. Coenuri Infection,Coenurosis,Coenurus Infection,Coenurus cerebralis Infection,Cysticercus cellulosae Infection,Taenia solium Cysticercosis,Coenuri Infections,Coenuroses,Coenurus Infections,Coenurus cerebralis Infections,Cysticercoses,Cysticercosis, Taenia solium,Cysticercus cellulosae Infections,Infection, Coenuri,Infection, Coenurus,Infection, Coenurus cerebralis,Infection, Cysticercus cellulosae,Taenia solium Cysticercoses
D003552 Cysticercus The larval form of various tapeworms of the genus Taenia.
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

C Retamal, and I Zulantay, and H Sariego, and R Melo, and W Apt
April 1986, Archives of neurology,
C Retamal, and I Zulantay, and H Sariego, and R Melo, and W Apt
January 1988, AJNR. American journal of neuroradiology,
C Retamal, and I Zulantay, and H Sariego, and R Melo, and W Apt
January 1990, Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases,
C Retamal, and I Zulantay, and H Sariego, and R Melo, and W Apt
January 1979, Lancet (London, England),
C Retamal, and I Zulantay, and H Sariego, and R Melo, and W Apt
January 2004, Revista do Instituto de Medicina Tropical de Sao Paulo,
C Retamal, and I Zulantay, and H Sariego, and R Melo, and W Apt
January 1996, Boletin chileno de parasitologia,
C Retamal, and I Zulantay, and H Sariego, and R Melo, and W Apt
April 1982, Annals of tropical medicine and parasitology,
C Retamal, and I Zulantay, and H Sariego, and R Melo, and W Apt
September 1985, The Southeast Asian journal of tropical medicine and public health,
C Retamal, and I Zulantay, and H Sariego, and R Melo, and W Apt
December 2008, Asian Pacific journal of allergy and immunology,
C Retamal, and I Zulantay, and H Sariego, and R Melo, and W Apt
July 1979, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie,
Copied contents to your clipboard!