BIOMAT Database Logo BIOMAT Database Logo

Decreased birefringence of the superficial zone collagen network in the canine knee (stifle) articular cartilage after long distance running training, detected by quantitative polarised light microscopy. 1996

J P Arokoski, and M M Hyttinen, and T Lapveteläinen, and P Takács, and B Kosztáczky, and L Módis, and V Kovanen, and H Helminen
Department of Physical and Rehabilitation Medicine, Kuopio University Hospital, Finland.

OBJECTIVE To investigate the effects of a one year programme of running training (up to 40 km/day for 15 weeks) on the spatial orientation pattern of collagen and glycosaminoglycans in articular cartilage in different parts of the knee (stifle) and shoulder joints of young beagle dogs. METHODS Area specific measurements of the optical path difference (= retardation, gamma) and the cartilage zone thickness were performed using conventional procedures and a new computer based quantitative polarised light microscopy method. Transmission electron microscopy was used to determine the zonal volume density of collagen fibrils. The concentrations of collagen and hydroxypyridinium crosslinks were investigated biochemically. RESULTS Running training decreased gamma by 24-34% (p < 0.05) in the superficial zone of the lateral femoral condyle articular cartilage and at the centre of the tibial condyles. Gamma of glycosaminoglycans decreased by 26% (p < 0.05) in the superficial zone of the lateral condyle of the femur, but at the same site the volume density of collagen fibrils was unchanged. Neither the collagen concentration nor the concentration of hydroxypyridinium crosslinks was altered as a result of running. In both control and runner dogs, the thickness and gamma values of the superficial zone were greater in the humerus and the femur than in the tibia. CONCLUSIONS Endurance type running exercise in beagles caused a reduction in the superficial zone birefringence of the articular cartilage, which indicates either a disorganisation or a reorientation of the superficial zone collagen network. Articular cartilage showed marked variability of collagen network organisation over the different knee (stifle) joint articular surfaces.

UI MeSH Term Description Entries
D007719 Knee Joint A synovial hinge connection formed between the bones of the FEMUR; TIBIA; and PATELLA. Superior Tibiofibular Joint,Joint, Knee,Joint, Superior Tibiofibular,Knee Joints,Superior Tibiofibular Joints,Tibiofibular Joint, Superior
D008859 Microscopy, Polarization Microscopy using polarized light in which phenomena due to the preferential orientation of optical properties with respect to the vibration plane of the polarized light are made visible and correlated parameters are made measurable. Microscopy, Polarized Light,Polarization Microscopy,Light Microscopies, Polarized,Light Microscopy, Polarized,Microscopies, Polarization,Microscopies, Polarized Light,Polarization Microscopies,Polarized Light Microscopies,Polarized Light Microscopy
D010805 Physical Conditioning, Animal Diet modification and physical exercise to improve the ability of animals to perform physical activities. Animal Physical Conditioning,Animal Physical Conditionings,Conditioning, Animal Physical,Conditionings, Animal Physical,Physical Conditionings, Animal
D002358 Cartilage, Articular A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact. Articular Cartilage,Articular Cartilages,Cartilages, Articular
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D004285 Dogs The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065) Canis familiaris,Dog
D005260 Female Females
D005269 Femur The longest and largest bone of the skeleton, it is situated between the hip and the knee. Trochanter,Greater Trochanter,Lesser Trochanter,Femurs,Greater Trochanters,Lesser Trochanters,Trochanter, Greater,Trochanter, Lesser,Trochanters,Trochanters, Greater,Trochanters, Lesser
D006025 Glycosaminoglycans Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine (see ACETYLGLUCOSAMINE) or N-acetylgalactosamine (see ACETYLGALACTOSAMINE). Glycosaminoglycan,Mucopolysaccharides
D006811 Humerus Bone in humans and primates extending from the SHOULDER JOINT to the ELBOW JOINT. Humeri,Greater Tubercle,Humeral Greater Tuberosity,Humeral Lesser Tuberosity,Lesser Tubercle,Olecranon Fossa,Olecranon Fossi,Greater Tubercles,Greater Tuberosities, Humeral,Greater Tuberosity, Humeral,Humeral Greater Tuberosities,Humeral Lesser Tuberosities,Lesser Tubercles,Lesser Tuberosities, Humeral,Lesser Tuberosity, Humeral,Tubercle, Greater,Tubercles, Greater

Related Publications

J P Arokoski, and M M Hyttinen, and T Lapveteläinen, and P Takács, and B Kosztáczky, and L Módis, and V Kovanen, and H Helminen
Softening of the lateral condyle articular cartilage in the canine knee joint after long distance (up to 40 km/day) running training lasting one year.
July 1994, International journal of sports medicine,
J P Arokoski, and M M Hyttinen, and T Lapveteläinen, and P Takács, and B Kosztáczky, and L Módis, and V Kovanen, and H Helminen
Polarized reflectance from articular cartilage depends upon superficial zone collagen network microstructure.
November 2019, Biomedical optics express,
J P Arokoski, and M M Hyttinen, and T Lapveteläinen, and P Takács, and B Kosztáczky, and L Módis, and V Kovanen, and H Helminen
Site-specific immunostaining for type X collagen in noncalcified articular cartilage of canine stifle knee joint.
December 2002, Bone,
J P Arokoski, and M M Hyttinen, and T Lapveteläinen, and P Takács, and B Kosztáczky, and L Módis, and V Kovanen, and H Helminen
The collagen fibril structure in the superficial zone of articular cartilage by microMRI.
November 2009, Osteoarthritis and cartilage,
J P Arokoski, and M M Hyttinen, and T Lapveteläinen, and P Takács, and B Kosztáczky, and L Módis, and V Kovanen, and H Helminen
Adaptation of canine femoral head articular cartilage to long distance running exercise in young beagles.
May 1993, Annals of the rheumatic diseases,
J P Arokoski, and M M Hyttinen, and T Lapveteläinen, and P Takács, and B Kosztáczky, and L Módis, and V Kovanen, and H Helminen
The superficial zone of articular cartilage.
May 2022, Inflammation and regeneration,
J P Arokoski, and M M Hyttinen, and T Lapveteläinen, and P Takács, and B Kosztáczky, and L Módis, and V Kovanen, and H Helminen
Proteoglycan synthesis in canine intervertebral discs after long-distance running training.
September 1993, Journal of orthopaedic research : official publication of the Orthopaedic Research Society,
J P Arokoski, and M M Hyttinen, and T Lapveteläinen, and P Takács, and B Kosztáczky, and L Módis, and V Kovanen, and H Helminen
Partial restoration of immobilization-induced softening of canine articular cartilage after remobilization of the knee (stifle) joint.
January 1989, Journal of orthopaedic research : official publication of the Orthopaedic Research Society,
J P Arokoski, and M M Hyttinen, and T Lapveteläinen, and P Takács, and B Kosztáczky, and L Módis, and V Kovanen, and H Helminen
Quantitative study of articular cartilage and subchondral bone remodeling in the knee joint of dogs after strenuous running training.
December 1992, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,
J P Arokoski, and M M Hyttinen, and T Lapveteläinen, and P Takács, and B Kosztáczky, and L Módis, and V Kovanen, and H Helminen
Articular cartilage thickness and glycosaminoglycan distribution in the canine knee joint after strenuous running exercise.
October 1992, Clinical orthopaedics and related research,
Copied contents to your clipboard!