BIOMAT Database Logo BIOMAT Database Logo

Rapid detection cytomegalovirus pneumonia in recipients of bone marrow transplant: evaluation and comparison of five survey methods for bronchoalveolar lavage fluid. 1996

H Goto, and K Yuasa, and H Sakamaki, and K Nakata, and I Komuro, and M Iguchi, and T Okamura, and R Ieki, and S Tanikawa, and H Akiyama, and Y Onozawa, and Y Mochida
Department of Internal Medicine, Tokyo Metropolitan Komagome General Hospital, Japan.

To detect cytomegalovirus-associated interstitial pneumonia (CMV-IP) in recipients of BMT in its earliest stage, five CMV methods were assessed for their usefulness using bronchoalveolar lavage fluid as the test specimen. Of the 43 cases enrolled in the study, PCR was positive in 12 cases, shell vial in eight, culture in eight and cytology in three. There were no positive cases in in situ hybridization. Based on this result, the 43 cases were classified into four groups: Group 1, three cases: positive in PCR, shell vial and cytology; Group 2, five cases: positive in PCR and shell vial; Group 3, four cases: positive only in PCR; and Group 4, 31 cases: negative in all CMV tests. Cases in Group 1 were judged as having the highest risk of overt CMV-IP. They were successfully treated with a combination of ganciclovir and immunoglobulin. Group 2 was diagnosed as having active CMV infection and ganciclovir monotherapy was effective for these patients. Groups 3 and 4 were not given anti-CMV therapy, but they were free from CMV-related manifestations throughout the study. The sensitivity and specificity of each survey method for the detection of Groups 1 and 2 were 1.0 and 0.89 in PCR, 1.0 and 1.0 in shell vial, 0.88 and 1.0 in culture, and 0.38 and 1.0 in cytology. Similarly, the positive and negative predictive values were 0.67 and 1.0 in PCR, 1.0 and 1.0 in shell vial, 1.0 and 0.97 in culture, and 1.0 and 0.88 in cytology. Thus, CMV survey on bronchoalveolar fluid was thought to be useful in detecting post BMT CMV-IP in its earliest stage.

UI MeSH Term Description Entries
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D011024 Pneumonia, Viral Inflammation of the lung parenchyma that is caused by a viral infection. Pneumonias, Viral,Viral Pneumonia,Viral Pneumonias
D001992 Bronchoalveolar Lavage Fluid Washing liquid obtained from irrigation of the lung, including the BRONCHI and the PULMONARY ALVEOLI. It is generally used to assess biochemical, inflammatory, or infection status of the lung. Alveolar Lavage Fluid,Bronchial Lavage Fluid,Lung Lavage Fluid,Bronchial Alveolar Lavage Fluid,Lavage Fluid, Bronchial,Lavage Fluid, Lung,Pulmonary Lavage Fluid,Alveolar Lavage Fluids,Bronchial Lavage Fluids,Bronchoalveolar Lavage Fluids,Lavage Fluid, Alveolar,Lavage Fluid, Bronchoalveolar,Lavage Fluid, Pulmonary,Lavage Fluids, Alveolar,Lavage Fluids, Bronchial,Lavage Fluids, Bronchoalveolar,Lavage Fluids, Lung,Lavage Fluids, Pulmonary,Lung Lavage Fluids,Pulmonary Lavage Fluids
D003586 Cytomegalovirus Infections Infection with CYTOMEGALOVIRUS, characterized by enlarged cells bearing intranuclear inclusions. Infection may be in almost any organ, but the salivary glands are the most common site in children, as are the lungs in adults. CMV Inclusion,CMV Inclusions,Congenital CMV Infection,Congenital Cytomegalovirus Infection,Cytomegalic Inclusion Disease,Cytomegalovirus Colitis,Cytomegalovirus Inclusion,Cytomegalovirus Inclusion Disease,Cytomegalovirus Inclusions,Inclusion Disease,Perinatal CMV Infection,Perinatal Cytomegalovirus Infection,Renal Tubular Cytomegalovirus Inclusion,Renal Tubular Cytomegalovirus Inclusions,Salivary Gland Virus Disease,Severe Cytomegalovirus Infection,Severe Cytomegalovirus Infections,Infections, Cytomegalovirus,CMV Infection, Congenital,CMV Infection, Perinatal,Colitis, Cytomegalovirus,Congenital CMV Infections,Congenital Cytomegalovirus Infections,Cytomegalic Inclusion Diseases,Cytomegalovirus Colitides,Cytomegalovirus Inclusion Diseases,Cytomegalovirus Infection,Cytomegalovirus Infection, Congenital,Cytomegalovirus Infection, Perinatal,Cytomegalovirus Infection, Severe,Cytomegalovirus Infections, Severe,Disease, Cytomegalic Inclusion,Disease, Cytomegalovirus Inclusion,Diseases, Cytomegalovirus Inclusion,Inclusion Disease, Cytomegalic,Inclusion Disease, Cytomegalovirus,Inclusion Diseases,Inclusion Diseases, Cytomegalovirus,Inclusion, CMV,Inclusion, Cytomegalovirus,Infection, Congenital CMV,Infection, Congenital Cytomegalovirus,Infection, Cytomegalovirus,Infection, Perinatal CMV,Infection, Perinatal Cytomegalovirus,Infection, Severe Cytomegalovirus,Perinatal CMV Infections,Perinatal Cytomegalovirus Infections
D003587 Cytomegalovirus A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS. Herpesvirus 5, Human,Human Herpesvirus 5,Salivary Gland Viruses,HHV 5,Herpesvirus 5 (beta), Human,Cytomegaloviruses,Salivary Gland Virus,Virus, Salivary Gland,Viruses, Salivary Gland
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D005069 Evaluation Studies as Topic Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

H Goto, and K Yuasa, and H Sakamaki, and K Nakata, and I Komuro, and M Iguchi, and T Okamura, and R Ieki, and S Tanikawa, and H Akiyama, and Y Onozawa, and Y Mochida
Cytomegalovirus is frequently isolated in bronchoalveolar lavage fluid of bone marrow transplant recipients without pneumonia.
June 1990, Annals of internal medicine,
H Goto, and K Yuasa, and H Sakamaki, and K Nakata, and I Komuro, and M Iguchi, and T Okamura, and R Ieki, and S Tanikawa, and H Akiyama, and Y Onozawa, and Y Mochida
Comparison of plasma PCR and bronchoalveolar lavage fluid culture for detection of cytomegalovirus infection in adult bone marrow transplant recipients.
September 1994, Journal of clinical microbiology,
H Goto, and K Yuasa, and H Sakamaki, and K Nakata, and I Komuro, and M Iguchi, and T Okamura, and R Ieki, and S Tanikawa, and H Akiyama, and Y Onozawa, and Y Mochida
Increased cytotoxicity against cytomegalovirus-infected target cells by bronchoalveolar lavage cells from bone marrow transplant recipients with cytomegalovirus pneumonia.
October 1988, The Journal of infectious diseases,
H Goto, and K Yuasa, and H Sakamaki, and K Nakata, and I Komuro, and M Iguchi, and T Okamura, and R Ieki, and S Tanikawa, and H Akiyama, and Y Onozawa, and Y Mochida
Clinical significance of quantitative cytomegalovirus detection in bronchoalveolar lavage fluid in lung transplant recipients.
February 2013, Transplant infectious disease : an official journal of the Transplantation Society,
H Goto, and K Yuasa, and H Sakamaki, and K Nakata, and I Komuro, and M Iguchi, and T Okamura, and R Ieki, and S Tanikawa, and H Akiyama, and Y Onozawa, and Y Mochida
Interleukin-2, interferon-gamma and natural killer cell activity in bronchoalveolar lavage fluid from marrow transplant recipients with cytomegalovirus pneumonia.
February 1993, Bone marrow transplantation,
H Goto, and K Yuasa, and H Sakamaki, and K Nakata, and I Komuro, and M Iguchi, and T Okamura, and R Ieki, and S Tanikawa, and H Akiyama, and Y Onozawa, and Y Mochida
Cytomegalovirus (CMV) DNA Quantitation in Bronchoalveolar Lavage Fluid From Hematopoietic Stem Cell Transplant Recipients With CMV Pneumonia.
May 2017, The Journal of infectious diseases,
H Goto, and K Yuasa, and H Sakamaki, and K Nakata, and I Komuro, and M Iguchi, and T Okamura, and R Ieki, and S Tanikawa, and H Akiyama, and Y Onozawa, and Y Mochida
Value of cytomegalovirus detection by PCR in bronchoalveolar lavage routinely performed in asymptomatic bone marrow recipients.
October 1997, Bone marrow transplantation,
H Goto, and K Yuasa, and H Sakamaki, and K Nakata, and I Komuro, and M Iguchi, and T Okamura, and R Ieki, and S Tanikawa, and H Akiyama, and Y Onozawa, and Y Mochida
Rapid detection of cytomegalovirus antigen on alveolar cells in bronchoalveolar fluid from transplant patients with cytomegalovirus pneumonia.
October 1994, Clinical transplantation,
H Goto, and K Yuasa, and H Sakamaki, and K Nakata, and I Komuro, and M Iguchi, and T Okamura, and R Ieki, and S Tanikawa, and H Akiyama, and Y Onozawa, and Y Mochida
Molecular methods for cytomegalovirus surveillance in bone marrow transplant recipients.
November 2002, Journal of clinical microbiology,
H Goto, and K Yuasa, and H Sakamaki, and K Nakata, and I Komuro, and M Iguchi, and T Okamura, and R Ieki, and S Tanikawa, and H Akiyama, and Y Onozawa, and Y Mochida
Cytomegalovirus pneumonia in bone marrow transplant recipients: miliary and diffuse patterns.
July 1980, The American review of respiratory disease,
Copied contents to your clipboard!