BIOMAT Database Logo BIOMAT Database Logo

Analysis of benzoylecgonine in dried blood spots by liquid chromatography--atmospheric pressure chemical ionization tandem mass spectrometry. 1996

C S Sosnoff, and Q Ann, and J T Bernert, and M K Powell, and B B Miller, and L O Henderson, and W H Hannon, and P Fernhoff, and E J Sampson
Division of Environmental Health Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, U.S. Public Health Service, Atlanta, GA 30333, USA.

Residual samples from blood spots (i.e., whole blood spotted onto filter paper) are a useful source for epidemiological screening studies involving newborns. However, the small volume of blood available from residual blood spots complicates the assay. A method for analyzing benzoylecgonine (BZE; the primary metabolite of cocaine) in blood spots, in which the blood spot is eluted with aqueous ammonium acetate-methanol containing N-methyl trideuterated-BZE as an internal standard, followed by high-performance liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry using multiple reaction monitoring, has been developed. This approach provides a rapid, direct, sensitive (limit of detection, approximately 2 ng/mL, based on a 12-microL sample size), and highly specific means of determining BZE concentrations in blood spots. We have applied this method for confirmatory analyses in a large epidemiological study of the prevalence of cocaine use during late pregnancy.

UI MeSH Term Description Entries
D007231 Infant, Newborn An infant during the first 28 days after birth. Neonate,Newborns,Infants, Newborn,Neonates,Newborn,Newborn Infant,Newborn Infants
D007553 Isotope Labeling Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms. Isotope Labeling, Stable,Isotope-Coded Affinity Tagging,Isotopically-Coded Affinity Tagging,Affinity Tagging, Isotope-Coded,Affinity Tagging, Isotopically-Coded,Isotope Coded Affinity Tagging,Labeling, Isotope,Labeling, Stable Isotope,Stable Isotope Labeling,Tagging, Isotope-Coded Affinity,Tagging, Isotopically-Coded Affinity
D008401 Gas Chromatography-Mass Spectrometry A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. Chromatography, Gas-Liquid-Mass Spectrometry,Chromatography, Gas-Mass Spectrometry,GCMS,Spectrometry, Mass-Gas Chromatography,Spectrum Analysis, Mass-Gas Chromatography,Gas-Liquid Chromatography-Mass Spectrometry,Mass Spectrometry-Gas Chromatography,Chromatography, Gas Liquid Mass Spectrometry,Chromatography, Gas Mass Spectrometry,Chromatography, Mass Spectrometry-Gas,Chromatography-Mass Spectrometry, Gas,Chromatography-Mass Spectrometry, Gas-Liquid,Gas Chromatography Mass Spectrometry,Gas Liquid Chromatography Mass Spectrometry,Mass Spectrometry Gas Chromatography,Spectrometries, Mass-Gas Chromatography,Spectrometry, Gas Chromatography-Mass,Spectrometry, Gas-Liquid Chromatography-Mass,Spectrometry, Mass Gas Chromatography,Spectrometry-Gas Chromatography, Mass,Spectrum Analysis, Mass Gas Chromatography
D008431 Maternal-Fetal Exchange Exchange of substances between the maternal blood and the fetal blood at the PLACENTA via PLACENTAL CIRCULATION. The placental barrier excludes microbial or viral transmission. Transplacental Exposure,Exchange, Maternal-Fetal,Exposure, Transplacental,Maternal Fetal Exchange
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D011248 Pregnancy Complications Conditions or pathological processes associated with pregnancy. They can occur during or after pregnancy, and range from minor discomforts to serious diseases that require medical interventions. They include diseases in pregnant females, and pregnancies in females with diseases. Adverse Birth Outcomes,Complications, Pregnancy,Adverse Birth Outcome,Birth Outcome, Adverse,Complication, Pregnancy,Outcome, Adverse Birth,Pregnancy Complication
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D012015 Reference Standards A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy. Standard Preparations,Standards, Reference,Preparations, Standard,Standardization,Standards,Preparation, Standard,Reference Standard,Standard Preparation,Standard, Reference
D002138 Calibration Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output. Calibrations
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance

Related Publications

C S Sosnoff, and Q Ann, and J T Bernert, and M K Powell, and B B Miller, and L O Henderson, and W H Hannon, and P Fernhoff, and E J Sampson
Analysis of methylcitrate in dried blood spots by liquid chromatography-tandem mass spectrometry.
January 2014, JIMD reports,
C S Sosnoff, and Q Ann, and J T Bernert, and M K Powell, and B B Miller, and L O Henderson, and W H Hannon, and P Fernhoff, and E J Sampson
Analysis of hydroxylated polybrominated diphenyl ether metabolites by liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry.
August 2010, Rapid communications in mass spectrometry : RCM,
C S Sosnoff, and Q Ann, and J T Bernert, and M K Powell, and B B Miller, and L O Henderson, and W H Hannon, and P Fernhoff, and E J Sampson
Analysis of pyridoquinoline derivatives by liquid chromatography/atmospheric pressure chemical ionization mass spectrometry.
January 2001, Rapid communications in mass spectrometry : RCM,
C S Sosnoff, and Q Ann, and J T Bernert, and M K Powell, and B B Miller, and L O Henderson, and W H Hannon, and P Fernhoff, and E J Sampson
A validated liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry method for quantitation of cocaine and benzoylecgonine in human plasma.
October 2001, Journal of analytical toxicology,
C S Sosnoff, and Q Ann, and J T Bernert, and M K Powell, and B B Miller, and L O Henderson, and W H Hannon, and P Fernhoff, and E J Sampson
Analysis of cyclosporin A in dried blood spots using liquid chromatography tandem mass spectrometry.
May 2009, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
C S Sosnoff, and Q Ann, and J T Bernert, and M K Powell, and B B Miller, and L O Henderson, and W H Hannon, and P Fernhoff, and E J Sampson
Capillary liquid chromatography-microchip atmospheric pressure chemical ionization-mass spectrometry.
July 2006, Lab on a chip,
C S Sosnoff, and Q Ann, and J T Bernert, and M K Powell, and B B Miller, and L O Henderson, and W H Hannon, and P Fernhoff, and E J Sampson
Liquid chromatography-electron capture-atmospheric pressure chemical ionization-mass spectrometry.
August 2005, Analytical and bioanalytical chemistry,
C S Sosnoff, and Q Ann, and J T Bernert, and M K Powell, and B B Miller, and L O Henderson, and W H Hannon, and P Fernhoff, and E J Sampson
Quantification of tripdiolide in human whole blood by liquid chromatography coupled with atmospheric pressure chemical ionization tandem mass spectrometry.
April 2007, Talanta,
C S Sosnoff, and Q Ann, and J T Bernert, and M K Powell, and B B Miller, and L O Henderson, and W H Hannon, and P Fernhoff, and E J Sampson
Analysis of urinary organic acids by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry.
December 1993, Journal of chromatography,
C S Sosnoff, and Q Ann, and J T Bernert, and M K Powell, and B B Miller, and L O Henderson, and W H Hannon, and P Fernhoff, and E J Sampson
Trace analysis of arthropod hormones by liquid chromatography-atmospheric pressure chemical ionization/mass spectrometry.
August 1996, Biochemical Society transactions,
Copied contents to your clipboard!