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Interleukin-8 (IL-8) is a neutrophil and T-lymphocyte chemotactic and activating factor. This cytokine is produced by many cell types including macrophages in response to a variety of microbial and non-microbial agents. In the present study, we determined the nucleotide sequence for bovine IL-8 cDNA. The amino acid sequence encoded by this cDNA shares 76 and 87% homology with the human and swine IL-8 proteins, respectively. Bovine IL-8 cDNA was expressed in Escherichia coli as a beta-galactosidase fusion protein. Western blotting demonstrates that this fusion protein, but not beta-galactosidase cross-reacts with monospecific anti-human IL-8 antiserum. We also studied the induction of IL-8 mRNA synthesis in bovine alveolar macrophages (BAM) stimulated with heat-killed Pasteurella haemolytica. IL-8 mRNA was induced in BAM as early as 1 h and was detectable at high levels 12 h post-stimulation with P. haemolytica. A dose titration of P. haemolytica and E. coli endotoxins showed that a relatively low level of P. haemolytica endotoxin induced high levels of bovine IL-8 mRNA. The significance of these findings in the pathogenesis of bovine pneumonia caused by P. haemolytica is discussed.
M A Morsey, and
Y Popowych, and
J Kowalski, and
G Gerlach, and
D Godson, and
M Campos, and
L A Babiuk
September 1994,
Cytokine,
M A Morsey, and
Y Popowych, and
J Kowalski, and
G Gerlach, and
D Godson, and
M Campos, and
L A Babiuk
December 2003,
The Journal of veterinary medical science,
M A Morsey, and
Y Popowych, and
J Kowalski, and
G Gerlach, and
D Godson, and
M Campos, and
L A Babiuk
May 1986,
Proceedings of the National Academy of Sciences of the United States of America,
M A Morsey, and
Y Popowych, and
J Kowalski, and
G Gerlach, and
D Godson, and
M Campos, and
L A Babiuk
September 2003,
Veterinary immunology and immunopathology,
M A Morsey, and
Y Popowych, and
J Kowalski, and
G Gerlach, and
D Godson, and
M Campos, and
L A Babiuk
January 2000,
DNA sequence : the journal of DNA sequencing and mapping,
M A Morsey, and
Y Popowych, and
J Kowalski, and
G Gerlach, and
D Godson, and
M Campos, and
L A Babiuk
November 2011,
Developmental and comparative immunology,
M A Morsey, and
Y Popowych, and
J Kowalski, and
G Gerlach, and
D Godson, and
M Campos, and
L A Babiuk
May 1986,
Proceedings of the National Academy of Sciences of the United States of America,
M A Morsey, and
Y Popowych, and
J Kowalski, and
G Gerlach, and
D Godson, and
M Campos, and
L A Babiuk
December 1987,
Veterinary immunology and immunopathology,
M A Morsey, and
Y Popowych, and
J Kowalski, and
G Gerlach, and
D Godson, and
M Campos, and
L A Babiuk
December 1993,
Veterinary immunology and immunopathology,
M A Morsey, and
Y Popowych, and
J Kowalski, and
G Gerlach, and
D Godson, and
M Campos, and
L A Babiuk
March 1998,
DNA sequence : the journal of DNA sequencing and mapping,
