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Two forms of highly purified liver microsomal cytochrome P-450, P-450LM2, and P-450LM4, have been titrated with standardized solutions of sodium dithionite under anaerobic conditions. Only 1 electron was consumed per hemin molecule, and reoxidation of the reduced heme is accompanied by the transfer of 1 electron to oxidizing agents such as cytochrome c, cytochrome b5, or potassium ferricyanide. The present results are in disagreement with earlier dithionite titrations and reoxidation experiments which indicate that liver microsomal cytochrome P-450 is a 2-electron acceptor, but are in accord with previous potentimetric titrations and product yield data which indicate that this hemeprotein is a 1-electron acceptor. The cause for the discrepancy between the present and previous results is not fully understood.
J A Peterson, and
R E White, and
Y Yasukochi, and
M L Coomes, and
D H O'Keeffe, and
R E Ebel, and
B S Masters, and
D P Ballou, and
M J Coon
May 1986,
Archives of biochemistry and biophysics,
J A Peterson, and
R E White, and
Y Yasukochi, and
M L Coomes, and
D H O'Keeffe, and
R E Ebel, and
B S Masters, and
D P Ballou, and
M J Coon
December 1976,
FEBS letters,
J A Peterson, and
R E White, and
Y Yasukochi, and
M L Coomes, and
D H O'Keeffe, and
R E Ebel, and
B S Masters, and
D P Ballou, and
M J Coon
September 1996,
The Biochemical journal,
J A Peterson, and
R E White, and
Y Yasukochi, and
M L Coomes, and
D H O'Keeffe, and
R E Ebel, and
B S Masters, and
D P Ballou, and
M J Coon
September 1975,
The Journal of biological chemistry,
J A Peterson, and
R E White, and
Y Yasukochi, and
M L Coomes, and
D H O'Keeffe, and
R E Ebel, and
B S Masters, and
D P Ballou, and
M J Coon
November 1984,
Archives of biochemistry and biophysics,
J A Peterson, and
R E White, and
Y Yasukochi, and
M L Coomes, and
D H O'Keeffe, and
R E Ebel, and
B S Masters, and
D P Ballou, and
M J Coon
September 1981,
The Journal of biological chemistry,
J A Peterson, and
R E White, and
Y Yasukochi, and
M L Coomes, and
D H O'Keeffe, and
R E Ebel, and
B S Masters, and
D P Ballou, and
M J Coon
September 1986,
FEBS letters,
J A Peterson, and
R E White, and
Y Yasukochi, and
M L Coomes, and
D H O'Keeffe, and
R E Ebel, and
B S Masters, and
D P Ballou, and
M J Coon
April 1986,
Archives of biochemistry and biophysics,
J A Peterson, and
R E White, and
Y Yasukochi, and
M L Coomes, and
D H O'Keeffe, and
R E Ebel, and
B S Masters, and
D P Ballou, and
M J Coon
January 1979,
Acta biologica et medica Germanica,
J A Peterson, and
R E White, and
Y Yasukochi, and
M L Coomes, and
D H O'Keeffe, and
R E Ebel, and
B S Masters, and
D P Ballou, and
M J Coon
September 1977,
Biochemical and biophysical research communications,
