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The presence and distribution of nitric oxide synthase (NOS)-immunoreactive nerve fibers associated with the guinea pig major cerebral arteries was studied by means of immunohistochemical, histochemical and ultrastructural techniques. Anterior arteries of the circle of Willis received a rich supply of perivascular nerve fibers containing NOS immunoreactivity while posteriorly localized arteries presented a moderate to sparse innervation. A double immunofluorescence staining technique revealed that NOS was localized in nerve fibers distinct from those displaying substance P or tyrosine hydroxylase. Combined immunofluorescence and histochemical staining of the same preparation indicated that NOS immunoreactivity was localized in putative cholinergic nerve fibers (identified by their acetylcholinesterase content) and that NADPH-diaphorase activity (a marker for NOS-containing neurons) was found in nerves which also possessed VIP immunoreactivity. The ultrastructural study revealed that NOS immunoreactivity was present in numerous nerve varicosities at the adventitial-medial border. These results suggest that NO and VIP co-exist in putative parasympathetic nerve fibers supplying the guinea pig cerebral arteries and may be release together in response to nervous stimulation.
C P Barroso, and
L Edvinsson, and
W Zhang, and
M Cunha e Sá, and
D R Springall, and
J M Polak, and
S Gulbenkian
August 2010,
Autonomic neuroscience : basic & clinical,
C P Barroso, and
L Edvinsson, and
W Zhang, and
M Cunha e Sá, and
D R Springall, and
J M Polak, and
S Gulbenkian
May 1995,
Hypertension (Dallas, Tex. : 1979),
C P Barroso, and
L Edvinsson, and
W Zhang, and
M Cunha e Sá, and
D R Springall, and
J M Polak, and
S Gulbenkian
September 1994,
Journal of the autonomic nervous system,
C P Barroso, and
L Edvinsson, and
W Zhang, and
M Cunha e Sá, and
D R Springall, and
J M Polak, and
S Gulbenkian
November 1995,
Brain research,
C P Barroso, and
L Edvinsson, and
W Zhang, and
M Cunha e Sá, and
D R Springall, and
J M Polak, and
S Gulbenkian
October 2000,
American journal of physiology. Heart and circulatory physiology,
C P Barroso, and
L Edvinsson, and
W Zhang, and
M Cunha e Sá, and
D R Springall, and
J M Polak, and
S Gulbenkian
January 1997,
The Journal of physiology,
C P Barroso, and
L Edvinsson, and
W Zhang, and
M Cunha e Sá, and
D R Springall, and
J M Polak, and
S Gulbenkian
July 1985,
Brain research,
C P Barroso, and
L Edvinsson, and
W Zhang, and
M Cunha e Sá, and
D R Springall, and
J M Polak, and
S Gulbenkian
May 1973,
Journal of anatomy,
C P Barroso, and
L Edvinsson, and
W Zhang, and
M Cunha e Sá, and
D R Springall, and
J M Polak, and
S Gulbenkian
June 1997,
European journal of pharmacology,
C P Barroso, and
L Edvinsson, and
W Zhang, and
M Cunha e Sá, and
D R Springall, and
J M Polak, and
S Gulbenkian
January 1992,
General pharmacology,
