-transparent.png){kind=logo}
Purification of subunit B of Shiga toxin using a synthetic trisaccharide-based affinity matrix. 1996
Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
The blood group P1 antigenic trisaccharide (3), which is the receptor-binding ligand of Shiga-like toxins, is synthesized in a spacer-equipped form (32) from 2-(trimethylsilyl)ethyl glucoside 5 and the 1-thiogalactoside building blocks 10 and 22 in a stereocontrolled, stepwise fashion. Covalent attachment of 32 to hydrazine group-containing agarose gel by reductive amination provided the P1 trisaccharide-containing affinity sorbent which was used for preparative scale isolation of subunit B of Shiga toxin.
Related Publications
V Pozsgay, and
L Trinh, and
J Shiloach, and
J B Robbins, and
A Donohue-Rolfe, and
S B Calderwood
December 1995,
Protein expression and purification,
V Pozsgay, and
L Trinh, and
J Shiloach, and
J B Robbins, and
A Donohue-Rolfe, and
S B Calderwood
September 1990,
Infection and immunity,
V Pozsgay, and
L Trinh, and
J Shiloach, and
J B Robbins, and
A Donohue-Rolfe, and
S B Calderwood
July 2001,
Protein expression and purification,
V Pozsgay, and
L Trinh, and
J Shiloach, and
J B Robbins, and
A Donohue-Rolfe, and
S B Calderwood
April 2011,
Molecular imaging,
V Pozsgay, and
L Trinh, and
J Shiloach, and
J B Robbins, and
A Donohue-Rolfe, and
S B Calderwood
December 2004,
Protein expression and purification,
V Pozsgay, and
L Trinh, and
J Shiloach, and
J B Robbins, and
A Donohue-Rolfe, and
S B Calderwood
June 1988,
Infection and immunity,
V Pozsgay, and
L Trinh, and
J Shiloach, and
J B Robbins, and
A Donohue-Rolfe, and
S B Calderwood
January 1986,
Annali Sclavo. Collana monografica,
V Pozsgay, and
L Trinh, and
J Shiloach, and
J B Robbins, and
A Donohue-Rolfe, and
S B Calderwood
January 2015,
Protein and peptide letters,
V Pozsgay, and
L Trinh, and
J Shiloach, and
J B Robbins, and
A Donohue-Rolfe, and
S B Calderwood
June 2010,
Protein and peptide letters,
V Pozsgay, and
L Trinh, and
J Shiloach, and
J B Robbins, and
A Donohue-Rolfe, and
S B Calderwood
December 2008,
Biology of the cell,
