[Detection of anti-Treponema pallidum antibodies with colloidal gold immunoprobes]. 1996

J D Burgos, and M Mendoza, and J Vinasco, and R Hernández
Laboratorio Biotecnológico, Universidad INCCA de Colombia, Bogotá, S. América.

BACKGROUND The aim of this study was to evaluate the usefulness of colloidal gold probes widely used in histochemistry and electronic microscopy for the serologic diagnosis of syphilis. METHODS One hundred eighty-nine sera which had been submitted to the traditional fluorescent trigenemic absorption (FTA-ABS) test and had later been tested with the new colloidal gold probe method (IAT-ABS). RESULTS Out of a total of 103 positive samples by FTA-ABS all were positive when tested by gold immunoprobes (sensitivity 100%). Eighty-six samples which were negative by immunofluorescence were all negative by IAT-ABS (specificity 100%). CONCLUSIONS The new method of colloidal gold probes with the current microscopy is useful for confirming serologic diagnosis of syphilis.

UI MeSH Term Description Entries
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000293 Adolescent A person 13 to 18 years of age. Adolescence,Youth,Adolescents,Adolescents, Female,Adolescents, Male,Teenagers,Teens,Adolescent, Female,Adolescent, Male,Female Adolescent,Female Adolescents,Male Adolescent,Male Adolescents,Teen,Teenager,Youths
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D000907 Antibodies, Bacterial Immunoglobulins produced in a response to BACTERIAL ANTIGENS. Bacterial Antibodies
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013588 Syphilis Serodiagnosis Serologic tests for syphilis. Kahn Test,Wassermann Reaction,Serodiagnosis, Syphilis,Reaction, Wassermann,Serodiagnoses, Syphilis,Syphilis Serodiagnoses,Test, Kahn
D014210 Treponema pallidum The causative agent of venereal and non-venereal syphilis as well as yaws.
D017979 Gold Colloid A suspension of metallic gold particles. Colloidal Gold,Colloid, Gold,Gold, Colloidal

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