BIOMAT Database Logo BIOMAT Database Logo

Enzyme-linked immunosorbent assay (ELISA) using a glycolipid antigen for the serodiagnosis of melioidosis. 1995

L V Phung, and Y Han, and S Oka, and H Hotta, and M D Smith, and P Theeparakun, and E Yabuuchi, and I Yano
Department of Bacteriology, Osaka City University Medical School, Abeno-ku, Japan.

The serodiagnosis of melioidosis is commonly performed with tests using protein or polysaccharide as antigen. However, due to the low sensitivity, specificity and difficulty in the preparation of the antigens, more simple, precise and reproducible diagnostic tests were required. A purified glycolipid antigen (GL) which is a specific lipid component of Burkholderia pseudomallei has been used in an ELISA. With this antigen, specific immunoglobulin G (IgG) was detected in 49 out of 50 melioidosis sera. IgG was also detected in 2 out of 185 (Japanese) and 16 out of 181 (Vietnamese) control sera. Thus, the sensitivity was 98.0%, and specificity was 98.9% and 91.1% in the Japanese and Vietnamese sera, respectively. When the ELISA and indirect haemagglutination (IHA) tests were combined, a sensitivity of 100% and specificity of 97.8% were achieved. The advantages of the glycolipid antigen are ease of preparation, stability, high sensitivity and specificity.

UI MeSH Term Description Entries
D008554 Melioidosis A disease of humans and animals that resembles GLANDERS. It is caused by BURKHOLDERIA PSEUDOMALLEI and may range from a dormant infection to a condition that causes multiple abscesses, PNEUMONIA and BACTEREMIA. Whitmore's Disease,Burkholderia pseudomallei Infection,Burkholderia pseudomallei Infections,Disease, Whitmore's,Infection, Burkholderia pseudomallei,Melioidoses,Whitmore Disease
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006017 Glycolipids Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (From IUPAC's webpage) Glycolipid
D006385 Hemagglutination Inhibition Tests Serologic tests in which a known quantity of antigen is added to the serum prior to the addition of a red cell suspension. Reaction result is expressed as the smallest amount of antigen which causes complete inhibition of hemagglutination. Hemagglutination Inhibition Test,Inhibition Test, Hemagglutination,Inhibition Tests, Hemagglutination,Test, Hemagglutination Inhibition,Tests, Hemagglutination Inhibition
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000942 Antigens, Bacterial Substances elaborated by bacteria that have antigenic activity. Bacterial Antigen,Bacterial Antigens,Antigen, Bacterial
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D012698 Serologic Tests Diagnostic procedures involving immunoglobulin reactions. Serodiagnosis,Serologic Test,Serological Tests,Test, Serologic,Tests, Serologic,Serodiagnoses,Serological Test,Test, Serological,Tests, Serological
D016957 Burkholderia pseudomallei A species of gram-negative, aerobic bacteria that causes MELIOIDOSIS. It has been isolated from soil and water in tropical regions, particularly Southeast Asia. Pseudomonas pseudomallei

Related Publications

L V Phung, and Y Han, and S Oka, and H Hotta, and M D Smith, and P Theeparakun, and E Yabuuchi, and I Yano
Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis.
January 2007, Clinical and vaccine immunology : CVI,
L V Phung, and Y Han, and S Oka, and H Hotta, and M D Smith, and P Theeparakun, and E Yabuuchi, and I Yano
Serodiagnosis of Q fever by enzyme-linked immunosorbent assay (ELISA).
November 1987, Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,
L V Phung, and Y Han, and S Oka, and H Hotta, and M D Smith, and P Theeparakun, and E Yabuuchi, and I Yano
Serodiagnosis of extrapulmonary tuberculosis by enzyme-linked immunosorbent assay (ELISA).
January 1999, Roumanian archives of microbiology and immunology,
L V Phung, and Y Han, and S Oka, and H Hotta, and M D Smith, and P Theeparakun, and E Yabuuchi, and I Yano
Serodiagnosis of tuberculous meningitis by enzyme-linked immunosorbent assay (ELISA).
January 1996, Roumanian archives of microbiology and immunology,
L V Phung, and Y Han, and S Oka, and H Hotta, and M D Smith, and P Theeparakun, and E Yabuuchi, and I Yano
Enzyme-linked immunosorbent assay (ELISA) with variable antigen for serodiagnosis of T. B. gambiense trypanosomiasis.
January 1978, Annales de la Societe belge de medecine tropicale,
L V Phung, and Y Han, and S Oka, and H Hotta, and M D Smith, and P Theeparakun, and E Yabuuchi, and I Yano
Enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of hydatid disease.
January 1980, The American journal of tropical medicine and hygiene,
L V Phung, and Y Han, and S Oka, and H Hotta, and M D Smith, and P Theeparakun, and E Yabuuchi, and I Yano
Serodiagnosis of porcine cysticercosis by enzyme-linked immunosorbent assay (ELISA) using fractionated antigens.
May 1987, Veterinary parasitology,
L V Phung, and Y Han, and S Oka, and H Hotta, and M D Smith, and P Theeparakun, and E Yabuuchi, and I Yano
Serodiagnosis of tissue dwelling parasites: application of a multi-antigen enzyme-linked immunosorbent assay (ELISA) for screening.
January 1982, Annales de la Societe belge de medecine tropicale,
L V Phung, and Y Han, and S Oka, and H Hotta, and M D Smith, and P Theeparakun, and E Yabuuchi, and I Yano
Serodiagnosis of melioidosis by a competitive enzyme-linked immunosorbent assay using a lipopolysaccharide-specific monoclonal antibody.
January 2005, Asian Pacific journal of allergy and immunology,
L V Phung, and Y Han, and S Oka, and H Hotta, and M D Smith, and P Theeparakun, and E Yabuuchi, and I Yano
Comparative evaluation of enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of dourine.
August 1991, Veterinary parasitology,
Copied contents to your clipboard!