Biomaterial Database

[The morphologic changes of outer hair cells induced by hypotonic, hypertonic medium and electromotility]. 1995

L Chen, and S Jiang, and R Gu

PLA General Hospital, Beijing.

For further understanding of biological features of cochlear outer hair cells, outer hair cells were isolated from guinea pig cochlea with micromechanical method and observed when the microosmotic concentration of their culture medium were changed. Outer hair cells shortened 3.14 +/- 0.76% (P < 0.01) within one minute after the osmotic concentration of the medium decreased to 280 mmol/L, accompanied with increase of cell diameter. Elongation of 3.34 +/- 1.09% (P < 0.05) of outer hair cells were observed after osmotic concentration of the medium was increased to 320 Os mmol/L, accompanied with decrease of cell diameter. Oscillatory elongation and shortening of outer hair cells and oscillatory displacements of intracellular organelles could be observed when square wave electrical potentials were applied across isolated outer hair cells. Hypertonic high potassium medium (K+ 100 mmol/L, 320 Osmmol/L) decreased the oscillatory movement of the cells by about 43.14% (negative potential) and 46.17% (positive potential) (P < 0.05). The shape of outer hair cells restored when the medium was isotonic. The mechanism and biological bases of outer hair cell movement were discussed.

UI	MeSH Term	Description	Entries
D006982	Hypertonic Solutions	Solutions that have a greater osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.	Hypertonic Solution,Solution, Hypertonic,Solutions, Hypertonic
D007038	Hypotonic Solutions	Solutions that have a lesser osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.	Solutions, Hypotonic
D008297	Male		Males
D009997	Osmotic Pressure	The pressure required to prevent the passage of solvent through a semipermeable membrane that separates a pure solvent from a solution of the solvent and solute or that separates different concentrations of a solution. It is proportional to the osmolality of the solution.	Osmotic Shock,Hypertonic Shock,Hypertonic Stress,Hypotonic Shock,Hypotonic Stress,Osmotic Stress,Hypertonic Shocks,Hypertonic Stresses,Hypotonic Shocks,Hypotonic Stresses,Osmotic Pressures,Osmotic Shocks,Osmotic Stresses,Pressure, Osmotic,Pressures, Osmotic,Shock, Hypertonic,Shock, Hypotonic,Shock, Osmotic,Shocks, Hypertonic,Shocks, Hypotonic,Shocks, Osmotic,Stress, Hypertonic,Stress, Hypotonic,Stress, Osmotic,Stresses, Hypertonic,Stresses, Hypotonic,Stresses, Osmotic
D011188	Potassium	An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
D002465	Cell Movement	The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.	Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D004558	Electric Stimulation	Use of electric potential or currents to elicit biological responses.	Stimulation, Electric,Electrical Stimulation,Electric Stimulations,Electrical Stimulations,Stimulation, Electrical,Stimulations, Electric,Stimulations, Electrical
D005260	Female		Females
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea