Biomaterial Database

Cloning and expression of a porcine UDP-GalNAc: polypeptide N-acetylgalactosaminyl transferase. 1995

A Yoshida, and T Hara, and H Ikenaga, and M Takeuchi

Central Laboratories for Key Technology, Kirin Brewery Co., Ltd, Fukuura, Japan.

By employing a bovine UDP-N-acetylgalactosamine: polypeptide N-acetylgalactosaminyl transferase (O-GalNAc transferase) cDNA as a probe, we isolated four overlapping cDNAs from a porcine lung cDNA library. Both the nucleotide sequence of the porcine cDNA and the predicted primary structure of the protein (559 amino acids) proved to be very similar to those of the bovine enzyme (95% and 99% identity, respectively). Transient expression of the clone in COS-7 cells, followed by enzymatic activity assays, demonstrated that this cDNA sequence encodes a porcine O-GalNAc transferase. The intracellular O-GalNAc transferase activity was increased approximately 100-fold by transfecting cells with the porcine cDNA.

UI	MeSH Term	Description	Entries
D008168	Lung	Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.	Lungs
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D000097763	Polypeptide N-acetylgalactosaminyltransferase	Family of enzymes that catalyze the formation of GalNAcAlpha1-serine/threonine linkages in glycoproteins.	Galactosylgalactosylglucosylceramide beta-D-acetylgalactosaminyltransferase,Globoside Synthase,Globoside beta GalNAc Transferase,Protein-UDPacetylgalactosaminyltransferase,(1-3)-N-acetyl-beta-galactosaminyltransferase,(1-4)-N-acetyl-beta-D-galactosaminyltransferase,4-GalNActransferase,GalNAc-T1,GalNAc-T10,GalNAc-T2,GalNAc-T3,GalNAc-T4,GalNAc-T5,GalNAc-T8,GalNAc-transferase,GalNAcT-1,GalNAcT-2,GalNAcT-4,GalNAcT-8,UDP-GPAGAT,UDP-GalNAc-beta-galactose beta 1,4-N-acetylgalactosaminyltransferase,UDP-GalNAc-polypeptide N-acetylgalactosaminyltransferase,UDP-N-acetyl-D-galactosamine polypeptide N-acetylgalactosaminyltransferase-T4,UDP-N-acetylgalactosamine mucin transferase,UDP-N-acetylgalactosamine-beta-galactose beta 1,4-N-acetylgalactosaminyltransferase,UDP-N-acetylgalactosamine-globoside beta-N-acetylgalactosaminyltransferase,UDP-N-acetylgalactosamine-globosidetriaosylceramide beta-3-N-acetylgalactosaminyltransferase,UDP-N-acetylgalactosamine-polypeptide N-acetylgalactosamine transferase,UDPacetylgalactosamine-galactosyl-galactosyl-glucosylceramide beta-N-acetyl-D-galactosaminyltransferase,UDPacetylgalactosamine-protein acetylgalactosaminyltransferase,beta-1,4-N-acetylgalactosaminyltransferase,beta-N-acetylgalactosaminyltransferase,beta1,6N-acetylgalactosaminyltransferase,polypeptide N-acetylgalactosaminyltransferase 1,polypeptide N-acetylgalactosaminyltransferase 10,polypeptide N-acetylgalactosaminyltransferase 2,polypeptide N-acetylgalactosaminyltransferase 3,polypeptide N-acetylgalactosaminyltransferase 4,polypeptide N-acetylgalactosaminyltransferase 5,polypeptide N-acetylgalactosaminyltransferase 8,pp-GalNAc-T10,ppGalNAc-T,Synthase, Globoside
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013552	Swine	Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).	Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D015183	Restriction Mapping	Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.	Endonuclease Mapping, Restriction,Enzyme Mapping, Restriction,Site Mapping, Restriction,Analysis, Restriction Enzyme,Enzyme Analysis, Restriction,Restriction Enzyme Analysis,Analyses, Restriction Enzyme,Endonuclease Mappings, Restriction,Enzyme Analyses, Restriction,Enzyme Mappings, Restriction,Mapping, Restriction,Mapping, Restriction Endonuclease,Mapping, Restriction Enzyme,Mapping, Restriction Site,Mappings, Restriction,Mappings, Restriction Endonuclease,Mappings, Restriction Enzyme,Mappings, Restriction Site,Restriction Endonuclease Mapping,Restriction Endonuclease Mappings,Restriction Enzyme Analyses,Restriction Enzyme Mapping,Restriction Enzyme Mappings,Restriction Mappings,Restriction Site Mapping,Restriction Site Mappings,Site Mappings, Restriction
D017350	N-Acetylgalactosaminyltransferases	Enzymes that catalyze the transfer of N-acetylgalactosamine from a nucleoside diphosphate N-acetylgalactosamine to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.	N-Acetylgalactosamine Transferases,N Acetylgalactosamine Transferases,N Acetylgalactosaminyltransferases,Transferases, N-Acetylgalactosamine