Biomaterial Database

Fluorescence energy transfer study of troponin C-melittin complex. 1995

H Sano, and T Iio

Department of Physics, Nagoya University, Chikusa-ku.

Fluorescence energy transfer study of rabbit skeletal troponin C (TnC), which binds a donor, dansylaziridine, to Met-25 in helix A and an acceptor, 5-(iodoacetamido)eosin, to Cys-98 in helix E revealed (i) the donor-acceptor distance did not change upon Mg2+ and Ca2+ binding to TnC, (ii) melittin binding to Ca(2+)-free TnC induced a remarkable decrease in the donor-acceptor distance both in the presence and absence of Mg2+, and (iii) Ca2+ binding to TnC in TnC-melittin complex induced a characteristic increase in the donor-acceptor distance. These results indicate that TnC assumes a contracted structure upon binding of the basic amphipathic TnC-binding peptide, melittin, and that it undergoes a characteristic structural change that includes a displacement of helix A away from helix E upon Ca2+ binding to TnC in the TnC-melittin complex.

UI	MeSH Term	Description	Entries
D008555	Melitten	Basic polypeptide from the venom of the honey bee (Apis mellifera). It contains 26 amino acids, has cytolytic properties, causes contracture of muscle, releases histamine, and disrupts surface tension, probably due to lysis of cell and mitochondrial membranes.	Melittin,Mellitin
D002135	Calcium-Binding Proteins	Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.	Calcium Binding Protein,Calcium-Binding Protein,Calcium Binding Proteins,Binding Protein, Calcium,Binding Proteins, Calcium,Protein, Calcium Binding,Protein, Calcium-Binding
D004735	Energy Transfer	The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.	Transfer, Energy
D013050	Spectrometry, Fluorescence	Measurement of the intensity and quality of fluorescence.	Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D015394	Molecular Structure	The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.	Structure, Molecular,Molecular Structures,Structures, Molecular
D019209	Troponin C	A TROPONIN complex subunit that binds calcium and help regulate calcium-dependent muscle contraction. There are two troponin C subtypes: troponin C1 and C2. Troponin C1 is skeletal and cardiac type whereas troponin C2 is skeletal type. Troponin C1 is a BIOMARKER for damaged or injured CARDIAC MYOCYTES and mutations in troponin C1 gene are associated with FAMILIAL HYPERTROPHIC CARDIOMYOPATHY.	Troponin C1,Troponin C2,Troponin-C