Antisense epidermal growth factor receptor transfection impairs the proliferative ability of human rhabdomyosarcoma cells. 1996

C De Giovanni, and L Landuzzi, and F Frabetti, and G Nicoletti, and C Griffoni, and I Rossi, and M Mazzotti, and L Scotto, and P Nanni, and P L Lollini
Cancer Research Institute, University of Bologna, Italy.

Human rhabdomyosarcoma cells express membrane epidermal growth factor receptor (ECF-R), which could confer responsiveness to EGF and transforming growth factor-alpha (TGF-alpha) of autocrine or paracrine origin. To study the role played by this growth factor circuit in the proliferation and differentiation of myogenic neoplastic cells, human rhabdomyosarcoma EGF-R-expressing cells (RD/18 clone) have been transfected with a plasmid containing a fragment of the EGF-R cDNA in the antisense orientation. In vitro growth and differentiative ability were studied on six antisense-transfected clones (AS) in comparison to parental RD/18 cells and to cells transfected with the plasmid containing only the neomycin resistance gene (NEO). A reduced EGF-R membrane expression was found in AS clones by decreased immunofluorescence with an anti-EGF-R monoclonal antibody. All AS transfectants had a greatly impaired proliferative ability, even when cultured in fetal bovine serum-containing medium. Proliferation of AS clones was completely blocked in medium supplemented with 2% horse serum. The differentiation ability of AS clones was heterogeneous, ranging from clones with a percentage of myosin-positive cells higher than controls to clones with a negligible myosin expression. Therefore, the growth impairment determined by the loop interruption is not sufficient to switch on the differentiation program. The role played by EGF-R in the proliferation of human rhabdomyosarcoma cells suggests that this receptor could constitute a target for a therapeutic approach.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012208 Rhabdomyosarcoma A malignant solid tumor arising from mesenchymal tissues which normally differentiate to form striated muscle. It can occur in a wide variety of sites. It is divided into four distinct types: pleomorphic, predominantly in male adults; alveolar (RHABDOMYOSARCOMA, ALVEOLAR), mainly in adolescents and young adults; embryonal (RHABDOMYOSARCOMA, EMBRYONAL), predominantly in infants and children; and botryoidal, also in young children. It is one of the most frequently occurring soft tissue sarcomas and the most common in children under 15. (From Dorland, 27th ed; Holland et al., Cancer Medicine, 3d ed, p2186; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, pp1647-9) Rhabdomyosarcomas
D014162 Transfection The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES. Transfections
D014407 Tumor Cells, Cultured Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured

Related Publications

C De Giovanni, and L Landuzzi, and F Frabetti, and G Nicoletti, and C Griffoni, and I Rossi, and M Mazzotti, and L Scotto, and P Nanni, and P L Lollini
April 2000, Molecular carcinogenesis,
C De Giovanni, and L Landuzzi, and F Frabetti, and G Nicoletti, and C Griffoni, and I Rossi, and M Mazzotti, and L Scotto, and P Nanni, and P L Lollini
June 2005, Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences,
C De Giovanni, and L Landuzzi, and F Frabetti, and G Nicoletti, and C Griffoni, and I Rossi, and M Mazzotti, and L Scotto, and P Nanni, and P L Lollini
April 2002, British journal of cancer,
C De Giovanni, and L Landuzzi, and F Frabetti, and G Nicoletti, and C Griffoni, and I Rossi, and M Mazzotti, and L Scotto, and P Nanni, and P L Lollini
October 1998, Neuropathology and applied neurobiology,
C De Giovanni, and L Landuzzi, and F Frabetti, and G Nicoletti, and C Griffoni, and I Rossi, and M Mazzotti, and L Scotto, and P Nanni, and P L Lollini
November 1998, Zhonghua yi xue za zhi,
C De Giovanni, and L Landuzzi, and F Frabetti, and G Nicoletti, and C Griffoni, and I Rossi, and M Mazzotti, and L Scotto, and P Nanni, and P L Lollini
January 1999, Breast cancer research and treatment,
C De Giovanni, and L Landuzzi, and F Frabetti, and G Nicoletti, and C Griffoni, and I Rossi, and M Mazzotti, and L Scotto, and P Nanni, and P L Lollini
August 2001, Electrophoresis,
C De Giovanni, and L Landuzzi, and F Frabetti, and G Nicoletti, and C Griffoni, and I Rossi, and M Mazzotti, and L Scotto, and P Nanni, and P L Lollini
January 2000, Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine,
C De Giovanni, and L Landuzzi, and F Frabetti, and G Nicoletti, and C Griffoni, and I Rossi, and M Mazzotti, and L Scotto, and P Nanni, and P L Lollini
June 2003, Zhonghua bing li xue za zhi = Chinese journal of pathology,
C De Giovanni, and L Landuzzi, and F Frabetti, and G Nicoletti, and C Griffoni, and I Rossi, and M Mazzotti, and L Scotto, and P Nanni, and P L Lollini
January 1998, Breast cancer research and treatment,
Copied contents to your clipboard!