-transparent.png){kind=logo}
Oxytocin has been previously shown to augment GnRH-stimulated LH release. However it is currently unknown which intracellular mediators participate in the process. In this study, after preincubation with oxytocin for 3 hours, quartered pituitaries were stimulated for 15 minutes with GnRH. The effects of diBucAMP, a cell permeable analog of cAMP, and DDA, an adenyl cyclase inhibitor, on the augmentation by oxytocin were investigated. Although addition of diBucAMP increased GnRH-stimulated LH release, it inhibited the augmentation by oxytocin of the response to GnRH. On the other hand addition of DDA induced an increased augmentation by oxytocin. These results indicate that intracellular cAMP inhibits the augmentory activity of oxytocin, and suggest that oxytocin modulation of GnRH action in vivo would be optimal when the hormonal milieu results in reduced levels of cAMP.
S J Hurd, and
J J Evans, and
D R Mason
June 1992,
General and comparative endocrinology,
S J Hurd, and
J J Evans, and
D R Mason
June 2001,
Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society,
S J Hurd, and
J J Evans, and
D R Mason
February 1989,
Endocrinology,
S J Hurd, and
J J Evans, and
D R Mason
January 1980,
The Journal of biological chemistry,
S J Hurd, and
J J Evans, and
D R Mason
June 1992,
Endocrinology,
S J Hurd, and
J J Evans, and
D R Mason
February 1987,
Endocrinology,
S J Hurd, and
J J Evans, and
D R Mason
April 1992,
Endocrinology,
S J Hurd, and
J J Evans, and
D R Mason
March 1979,
Biochemical and biophysical research communications,
S J Hurd, and
J J Evans, and
D R Mason
January 2009,
Neuroendocrinology,
S J Hurd, and
J J Evans, and
D R Mason
October 1991,
Proceedings of the National Academy of Sciences of the United States of America,
