Biomaterial Database

Symmetry and pH dependency of the lactate/proton carrier in skeletal muscle studied with rat sarcolemmal giant vesicles. 1996

C Juel

Copenhagen Muscle Research Centre. August Krogh Institute, University of Copenhagen, Denmark. CJuel@aki.ku.dk

Muscle lactate transport was studied in rat sarcolemmal giant vesicles, in which the membrane orientation was similar to that of intact cells. The lactate concentration dependency was studied using influx experiments under equilibrium exchange. zero-trans. and infinite-cis conditions. At constant external and internal pH the carrier behaved symmetrically with regard to lactate transport from the two sides of the membrane. Lactate at the trans side stimulated the lactate tracer flux (trans-acceleration). Both in zero-trans influx and efflux experiments the lactate flux was stimulated in a symmetric way by H+ at the cis side, consistent with protons being the substrate for the carrier. In contrast, the lactate flux was symmetrically inhibited by H+ at the trans side (trans-inhibition), the underlying mechanism is likely that the protonated carrier with no lactate bound is unable to reorient in the membrane. These findings have implications both for the kinetic model and for understanding the physiological function of the lactate/proton transporter.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D007773	Lactates	Salts or esters of LACTIC ACID containing the general formula CH3CHOHCOOR.
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001693	Biological Transport, Active	The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.	Active Transport,Uphill Transport,Active Biological Transport,Biologic Transport, Active,Transport, Active Biological,Active Biologic Transport,Transport, Active,Transport, Active Biologic,Transport, Uphill
D012508	Sarcolemma	The excitable plasma membrane of a muscle cell. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)	Sarcolemmas
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D018482	Muscle, Skeletal	A subtype of striated muscle, attached by TENDONS to the SKELETON. Skeletal muscles are innervated and their movement can be consciously controlled. They are also called voluntary muscles.	Anterior Tibial Muscle,Gastrocnemius Muscle,Muscle, Voluntary,Plantaris Muscle,Skeletal Muscle,Soleus Muscle,Muscle, Anterior Tibial,Muscle, Gastrocnemius,Muscle, Plantaris,Muscle, Soleus,Muscles, Skeletal,Muscles, Voluntary,Skeletal Muscles,Tibial Muscle, Anterior,Voluntary Muscle,Voluntary Muscles