BIOMAT Database Logo BIOMAT Database Logo

Molecular cloning of rat leptin receptor isoform complementary DNAs--identification of a missense mutation in Zucker fatty (fa/fa) rats. 1996

K Takaya, and Y Ogawa, and N Isse, and T Okazaki, and N Satoh, and H Masuzaki, and K Mori, and N Tamura, and K Hosoda, and K Nakao
Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Japan.

We cloned the full-length rat leptin receptor (Ob-R) isoform complementary DNAs (cDNAs) and examined the gene expression in rats. We also identified a mutation in Ob-R in Zucker fatty (fa/fa) rats. Three alternatively spliced isoforms (Ob-Ra, Ob-Rb, and Ob-Re) have been identified, which are closely related to the gp130 signal-transduction component of class I cytokine receptors. Rat Ob-Ra and Ob-Rb were single transmembrane proteins, which differ in the C-terminal amino acid sequences. On the other hand, Ob-Re had no transmembrane domain and was a soluble form of the receptor. Reverse transcription-polymerase chain reaction analysis revealed that Ob-R isoform messenger RNAs (mRNAs) are expressed in a wide variety of rat tissues in tissue-specific manners. A missense mutation (an A to C conversion at nucleotide position 806) was found in the extracellular domain of all the isoforms in Zucker fatty (fa/fa) rats, which resulted in an amino acid change from Gln to Pro at + 269 (the Gln269Pro mutation). These Ob-R isoform mRNAs were present in the brain from Zucker fatty (fa/fa) rats at comparable amounts to those in their lean littermates. The present study provides new insight into the molecular mechanisms for Ob-R.

UI MeSH Term Description Entries
D008297 Male Males
D008820 Mice, Obese Mutant mice exhibiting a marked obesity coupled with overeating, hyperglycemia, hyperinsulinemia, marked insulin resistance, and infertility when in a homozygous state. They may be inbred or hybrid. Hyperglycemic Mice,Obese Mice,Mouse, Hyperglycemic,Mouse, Obese,Hyperglycemic Mouse,Mice, Hyperglycemic,Obese Mouse
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009765 Obesity A status with BODY WEIGHT that is grossly above the recommended standards, usually due to accumulation of excess FATS in the body. The standards may vary with age, sex, genetic or cultural background. In the BODY MASS INDEX, a BMI greater than 30.0 kg/m2 is considered obese, and a BMI greater than 40.0 kg/m2 is considered morbidly obese (MORBID OBESITY).
D009928 Organ Specificity Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen. Tissue Specificity,Organ Specificities,Specificities, Organ,Specificities, Tissue,Specificity, Organ,Specificity, Tissue,Tissue Specificities
D011924 Rats, Zucker Two populations of Zucker rats have been cited in research--the "fatty" or obese and the lean. The "fatty" rat (Rattus norvegicus) appeared as a spontaneous mutant. The obese condition appears to be due to a single recessive gene. Zucker Rat,Zucker Rats,Rat, Zucker
D011956 Receptors, Cell Surface Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands. Cell Surface Receptor,Cell Surface Receptors,Hormone Receptors, Cell Surface,Receptors, Endogenous Substances,Cell Surface Hormone Receptors,Endogenous Substances Receptors,Receptor, Cell Surface,Surface Receptor, Cell
D002352 Carrier Proteins Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes. Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

K Takaya, and Y Ogawa, and N Isse, and T Okazaki, and N Satoh, and H Masuzaki, and K Mori, and N Tamura, and K Hosoda, and K Nakao
Leptin receptor missense mutation in the fatty Zucker rat.
May 1996, Nature genetics,
K Takaya, and Y Ogawa, and N Isse, and T Okazaki, and N Satoh, and H Masuzaki, and K Mori, and N Tamura, and K Hosoda, and K Nakao
Molecular cloning of rat obese cDNA and augmented gene expression in genetically obese Zucker fatty (fa/fa) rats.
September 1995, The Journal of clinical investigation,
K Takaya, and Y Ogawa, and N Isse, and T Okazaki, and N Satoh, and H Masuzaki, and K Mori, and N Tamura, and K Hosoda, and K Nakao
Phenotype-linked amino acid alteration in leptin receptor cDNA from Zucker fatty (fa/fa) rat.
May 1996, Biochemical and biophysical research communications,
K Takaya, and Y Ogawa, and N Isse, and T Okazaki, and N Satoh, and H Masuzaki, and K Mori, and N Tamura, and K Hosoda, and K Nakao
Molecular cloning of rat uncoupling protein 2 cDNA and its expression in genetically obese Zucker fatty (fa/fa) rats.
January 1998, Biochimica et biophysica acta,
K Takaya, and Y Ogawa, and N Isse, and T Okazaki, and N Satoh, and H Masuzaki, and K Mori, and N Tamura, and K Hosoda, and K Nakao
Characterization of bone structure in leptin receptor-deficient Zucker (fa/fa) rats.
September 2003, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,
K Takaya, and Y Ogawa, and N Isse, and T Okazaki, and N Satoh, and H Masuzaki, and K Mori, and N Tamura, and K Hosoda, and K Nakao
Detecting fa leptin receptor mutation in Zucker rats with tetra-primer amplification-refractory mutation system (ARMS)-PCR.
September 2023, Heliyon,
K Takaya, and Y Ogawa, and N Isse, and T Okazaki, and N Satoh, and H Masuzaki, and K Mori, and N Tamura, and K Hosoda, and K Nakao
Substitution at codon 269 (glutamine --> proline) of the leptin receptor (OB-R) cDNA is the only mutation found in the Zucker fatty (fa/fa) rat.
July 1996, Biochemical and biophysical research communications,
K Takaya, and Y Ogawa, and N Isse, and T Okazaki, and N Satoh, and H Masuzaki, and K Mori, and N Tamura, and K Hosoda, and K Nakao
Fever responses of Zucker rats with and without fatty mutation of the leptin receptor.
January 2002, American journal of physiology. Regulatory, integrative and comparative physiology,
K Takaya, and Y Ogawa, and N Isse, and T Okazaki, and N Satoh, and H Masuzaki, and K Mori, and N Tamura, and K Hosoda, and K Nakao
Supplemental fructose attenuates postprandial glycemia in Zucker fatty fa/fa rats.
June 2002, The Journal of nutrition,
K Takaya, and Y Ogawa, and N Isse, and T Okazaki, and N Satoh, and H Masuzaki, and K Mori, and N Tamura, and K Hosoda, and K Nakao
Leptin responsiveness and gene dosage for leptin receptor mutation (fa) in newborn rats.
May 1999, The American journal of physiology,
Copied contents to your clipboard!