BIOMAT Database Logo BIOMAT Database Logo

Evoked acetylcholine release expressed in neuroblastoma cells by transfection of mediatophore cDNA. 1996

J Falk-Vairant, and P Corrèges, and L Eder-Colli, and N Salem, and F M Meunier, and B Lesbats, and F Loctin, and M Synguelakis, and M Israël, and Y Dunant
Département de Pharmacologie, CMU, Geneva, Switzerland.

Transmitter release was elicited in two ways from cultured cells filled with acetylcholine: (a) in a biochemical assay by successive addition of a calcium ionophore and calcium and (b) electrophysiologically, by electrical stimulation of individual cells and real-time recording with an embryonic Xenopus myocyte. Glioma C6-Bu-1 cells were found to be competent for Ca(2+)-dependent and quantal release. In contrast, no release could be elicited from mouse neuroblastoma N18TG-2 cells. However, acetylcholine release could be restored when N18TG-2 cells were transfected with a plasmid coding for mediatophore. Mediatophore is a protein of nerve terminal membranes purified from the Torpedo electric organ on the basis of its acetylcholine-releasing capacity. The transfected N18TG-2 cells expressed Torpedo mediatophore in their plasma membrane. In response to an electrical stimulus, they generated in the myocyte evoked currents that were curare sensitive and calcium dependent and displayed, discrete amplitude levels, like in naturally occurring synapses.

UI MeSH Term Description Entries
D009419 Nerve Tissue Proteins Proteins, Nerve Tissue,Tissue Proteins, Nerve
D009447 Neuroblastoma A common neoplasm of early childhood arising from neural crest cells in the sympathetic nervous system, and characterized by diverse clinical behavior, ranging from spontaneous remission to rapid metastatic progression and death. This tumor is the most common intraabdominal malignancy of childhood, but it may also arise from thorax, neck, or rarely occur in the central nervous system. Histologic features include uniform round cells with hyperchromatic nuclei arranged in nests and separated by fibrovascular septa. Neuroblastomas may be associated with the opsoclonus-myoclonus syndrome. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2099-2101; Curr Opin Oncol 1998 Jan;10(1):43-51) Neuroblastomas
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D004558 Electric Stimulation Use of electric potential or currents to elicit biological responses. Stimulation, Electric,Electrical Stimulation,Electric Stimulations,Electrical Stimulations,Stimulation, Electrical,Stimulations, Electric,Stimulations, Electrical
D004594 Electrophysiology The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D005910 Glioma Benign and malignant central nervous system neoplasms derived from glial cells (i.e., astrocytes, oligodendrocytes, and ependymocytes). Astrocytes may give rise to astrocytomas (ASTROCYTOMA) or glioblastoma multiforme (see GLIOBLASTOMA). Oligodendrocytes give rise to oligodendrogliomas (OLIGODENDROGLIOMA) and ependymocytes may undergo transformation to become EPENDYMOMA; CHOROID PLEXUS NEOPLASMS; or colloid cysts of the third ventricle. (From Escourolle et al., Manual of Basic Neuropathology, 2nd ed, p21) Glial Cell Tumors,Malignant Glioma,Mixed Glioma,Glial Cell Tumor,Glioma, Malignant,Glioma, Mixed,Gliomas,Gliomas, Malignant,Gliomas, Mixed,Malignant Gliomas,Mixed Gliomas,Tumor, Glial Cell,Tumors, Glial Cell
D000109 Acetylcholine A neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system. 2-(Acetyloxy)-N,N,N-trimethylethanaminium,Acetilcolina Cusi,Acetylcholine Bromide,Acetylcholine Chloride,Acetylcholine Fluoride,Acetylcholine Hydroxide,Acetylcholine Iodide,Acetylcholine L-Tartrate,Acetylcholine Perchlorate,Acetylcholine Picrate,Acetylcholine Picrate (1:1),Acetylcholine Sulfate (1:1),Bromoacetylcholine,Chloroacetylcholine,Miochol,Acetylcholine L Tartrate,Bromide, Acetylcholine,Cusi, Acetilcolina,Fluoride, Acetylcholine,Hydroxide, Acetylcholine,Iodide, Acetylcholine,L-Tartrate, Acetylcholine,Perchlorate, Acetylcholine
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014101 Torpedo A genus of the Torpedinidae family consisting of several species. Members of this family have powerful electric organs and are commonly called electric rays. Electric Rays,Torpedinidae,Rays, Electric
D014162 Transfection The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES. Transfections

Related Publications

J Falk-Vairant, and P Corrèges, and L Eder-Colli, and N Salem, and F M Meunier, and B Lesbats, and F Loctin, and M Synguelakis, and M Israël, and Y Dunant
Mediatophore regulates acetylcholine release from T cells.
March 2012, Journal of neuroimmunology,
J Falk-Vairant, and P Corrèges, and L Eder-Colli, and N Salem, and F M Meunier, and B Lesbats, and F Loctin, and M Synguelakis, and M Israël, and Y Dunant
Acetylcholine synthesis and quantal release reconstituted by transfection of mediatophore and choline acetyltranferase cDNAs.
May 1999, The European journal of neuroscience,
J Falk-Vairant, and P Corrèges, and L Eder-Colli, and N Salem, and F M Meunier, and B Lesbats, and F Loctin, and M Synguelakis, and M Israël, and Y Dunant
Reconstitution of mediatophore-supported quantal acetylcholine release.
March 2000, Metabolic brain disease,
J Falk-Vairant, and P Corrèges, and L Eder-Colli, and N Salem, and F M Meunier, and B Lesbats, and F Loctin, and M Synguelakis, and M Israël, and Y Dunant
Mediatophore, a protein supporting quantal acetylcholine release.
September 1999, Canadian journal of physiology and pharmacology,
J Falk-Vairant, and P Corrèges, and L Eder-Colli, and N Salem, and F M Meunier, and B Lesbats, and F Loctin, and M Synguelakis, and M Israël, and Y Dunant
Increase in neurite formation and acetylcholine release by transfection of growth-associated protein-43 cDNA into NG108-15 cells.
August 1993, Journal of neurochemistry,
J Falk-Vairant, and P Corrèges, and L Eder-Colli, and N Salem, and F M Meunier, and B Lesbats, and F Loctin, and M Synguelakis, and M Israël, and Y Dunant
Ultrastructure and biophysics of acetylcholine release: central role of the mediatophore.
January 1993, Journal of physiology, Paris,
J Falk-Vairant, and P Corrèges, and L Eder-Colli, and N Salem, and F M Meunier, and B Lesbats, and F Loctin, and M Synguelakis, and M Israël, and Y Dunant
Properties of human interleukin-2 receptors expressed on non-lymphoid cells by cDNA transfection.
December 1984, Molecular biology & medicine,
J Falk-Vairant, and P Corrèges, and L Eder-Colli, and N Salem, and F M Meunier, and B Lesbats, and F Loctin, and M Synguelakis, and M Israël, and Y Dunant
Enhancement of quantal transmitter release and mediatophore expression by cyclic AMP in fibroblasts loaded with acetylcholine.
November 1996, Neuroscience,
J Falk-Vairant, and P Corrèges, and L Eder-Colli, and N Salem, and F M Meunier, and B Lesbats, and F Loctin, and M Synguelakis, and M Israël, and Y Dunant
Antisense agrin cDNA transfection blocks neuroblastoma cell-induced acetylcholine receptor aggregation when co-cultured with myotubes.
January 1997, Molecular and cellular neurosciences,
J Falk-Vairant, and P Corrèges, and L Eder-Colli, and N Salem, and F M Meunier, and B Lesbats, and F Loctin, and M Synguelakis, and M Israël, and Y Dunant
Characteristics of histamine release evoked by acetylcholine in isolated rat mast cells.
April 1980, The Journal of physiology,
Copied contents to your clipboard!