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The aim of this study was to determine whether a relationship exists between the circulating concentration of antioxidants, or markers of oxidative stress, and pulmonary function in cystic fibrosis patients. Plasma was obtained from 34 patients attending a cystic fibrosis clinic. Oxidative stress was investigated by measuring the concentrations of circulating lipid hydroperoxides and malondialdehyde (lipid peroxidation) and protein carbonyls (protein oxidation). Antioxidant status was determined from the plasma concentrations of alpha-tocopherol, ascorbic acid, uric acid and total sulphydryls. Forced vital capacity (FVC), forced expiratory volume in one second (FEV1) and forced mid-expiratory flow (FEF25-75) were measured in 25 of the subjects by spirometry, and expressed as percentage predicted for normal height, weight and age. Lung function decreased significantly with age and was associated with decreased plasma alpha-tocopherol, ascorbic acid and sulphydryl concentrations. The reduction in pulmonary function correlated with elevated plasma malondialdehyde, but not with lipid hydroperoxide or protein carbonyl concentrations. Patients with severe lung dysfunction (FEV1 < 50% predicted) had higher plasma concentrations of lipid hydroperoxides than those with mild-to-moderate lung dysfunction (FEV1 > 50% pred). This study provides evidence that cystic fibrosis patients have inadequate antioxidant defences to cope with the elevated oxidative stress that they regularly experience. We believe that recurring oxidative lung injury contributes to the decline in pulmonary function in these patients.
R K Brown, and
H Wyatt, and
J F Price, and
F J Kelly
July 2015,
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society,
R K Brown, and
H Wyatt, and
J F Price, and
F J Kelly
January 2019,
Oxidative medicine and cellular longevity,
R K Brown, and
H Wyatt, and
J F Price, and
F J Kelly
June 1997,
Thorax,
R K Brown, and
H Wyatt, and
J F Price, and
F J Kelly
November 2009,
The Clinical biochemist. Reviews,
R K Brown, and
H Wyatt, and
J F Price, and
F J Kelly
March 2020,
Comprehensive Physiology,
R K Brown, and
H Wyatt, and
J F Price, and
F J Kelly
March 2021,
Biomedicines,
R K Brown, and
H Wyatt, and
J F Price, and
F J Kelly
July 2013,
Biochimica et biophysica acta,
R K Brown, and
H Wyatt, and
J F Price, and
F J Kelly
February 2014,
Annals of the American Thoracic Society,
R K Brown, and
H Wyatt, and
J F Price, and
F J Kelly
May 2012,
Biochimica et biophysica acta,
R K Brown, and
H Wyatt, and
J F Price, and
F J Kelly
January 1995,
Nutrition (Burbank, Los Angeles County, Calif.),
