Masculine sexual behavior in rats can be stimulated by acutely stressing the animals. Nevertheless, little is known about the effect that different stressors could have on sexual behavior. In this work we studied the effect of different stressors applied both acutely and chronically on masculine sexual behavior. Sexually active male Wistar rats were submitted to stress by: immobilization (IMB); immersion in cold water (WIM); and electrical foot shock (EFS). These stressors were applied during 20 consecutive days and masculine sexual behavior was assessed on days 1, 4, 8, 12, 15 and 20. Motivational component, copulatory performance and copulatory potential were drastically altered by WIM. EFS produced significant alterations in almost all sexual parameters recorded but only when it was applied chronically. IMB only altered mount frequency and hit rate, although inconsistently. These results suggest that the effect of stress on sexual behavior depends on the nature and, in some conditions, on the duration of the stressor.