-transparent.png){kind=logo}
An immunoassay performed using a microchip electrophoretic system is described. Separation and quantitation of free and bound labeled antigen in a competitive assay are carried out in channels micromachined into fused silica substrates. Such microchips are attractive because of their small size, ruggedness, and amenability to automated handling. The assay achieves the determination of cortisol in blood serum over the range of clinical interest (1-60 micrograms/dL) without the need for extraction or other sample preparation steps. The separation is performed in less than 30 s. Very high throughput is possible by operating the assay in multiple channels in parallel. These characteristics make microchip electrophoretic systems a promising technology for the rapid analysis of clinical samples.
L B Koutny, and
D Schmalzing, and
T A Taylor, and
M Fuchs
March 1998,
Clinical chemistry,
L B Koutny, and
D Schmalzing, and
T A Taylor, and
M Fuchs
June 1992,
Clinica chimica acta; international journal of clinical chemistry,
L B Koutny, and
D Schmalzing, and
T A Taylor, and
M Fuchs
August 2002,
Journal of chromatography. A,
L B Koutny, and
D Schmalzing, and
T A Taylor, and
M Fuchs
January 1978,
Steroids,
L B Koutny, and
D Schmalzing, and
T A Taylor, and
M Fuchs
February 1995,
Analytical chemistry,
L B Koutny, and
D Schmalzing, and
T A Taylor, and
M Fuchs
August 1980,
Steroids,
L B Koutny, and
D Schmalzing, and
T A Taylor, and
M Fuchs
September 1995,
Analytical chemistry,
L B Koutny, and
D Schmalzing, and
T A Taylor, and
M Fuchs
June 2009,
Bulletin of experimental biology and medicine,
L B Koutny, and
D Schmalzing, and
T A Taylor, and
M Fuchs
August 2010,
Clinica chimica acta; international journal of clinical chemistry,
L B Koutny, and
D Schmalzing, and
T A Taylor, and
M Fuchs
January 2011,
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,
