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Prostaglandin E2 (PGE2) is an important regulator of systemic hemodynamics and epithelial ion transport. To further investigate the mechanism of PGE2 action, a clone encoding a PGE2 receptor was isolated from a rabbit kidney cortex cDNA library. Expression of the full-length cDNA in COS-1 cells yielded a ligand-binding profile typical for a butaprost-insensitive Gs-coupled E-prostanoid (EP) receptor. Misoprostol-free acid, a receptor-selective PGE analogue, produced concentration-dependent increases in adenosine 3',5'-cyclic monophosphate production. The data are consistent with the receptor being an EP4 subtype. Ribonuclease protection assays demonstrated that this receptor gene is highly expressed in intestine, uterus, and thymus, with lower but significant expression in kidney, whole adrenal, lung, spleen, and stomach. In situ hybridization in kidney revealed intense hybridization to glomeruli and urothelium of the renal pelvis. This prostanoid receptor was also highly expressed in the duodenal epithelium and adrenal cortex. The tissue distribution suggests a functional role for this receptor in mediating glomerular effects of PGE2 and effects on aldosterone secretion, intestinal transport, and immune function.
R M Breyer, and
L S Davis, and
C Nian, and
R Redha, and
B Stillman, and
H R Jacobson, and
M D Breyer
July 2001,
Prostaglandins & other lipid mediators,
R M Breyer, and
L S Davis, and
C Nian, and
R Redha, and
B Stillman, and
H R Jacobson, and
M D Breyer
March 2022,
Veterinary and comparative oncology,
R M Breyer, and
L S Davis, and
C Nian, and
R Redha, and
B Stillman, and
H R Jacobson, and
M D Breyer
June 2020,
BMC veterinary research,
R M Breyer, and
L S Davis, and
C Nian, and
R Redha, and
B Stillman, and
H R Jacobson, and
M D Breyer
November 2000,
Archives of otolaryngology--head & neck surgery,
R M Breyer, and
L S Davis, and
C Nian, and
R Redha, and
B Stillman, and
H R Jacobson, and
M D Breyer
January 2021,
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire,
R M Breyer, and
L S Davis, and
C Nian, and
R Redha, and
B Stillman, and
H R Jacobson, and
M D Breyer
June 2008,
General and comparative endocrinology,
R M Breyer, and
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C Nian, and
R Redha, and
B Stillman, and
H R Jacobson, and
M D Breyer
December 1999,
Biochemical pharmacology,
R M Breyer, and
L S Davis, and
C Nian, and
R Redha, and
B Stillman, and
H R Jacobson, and
M D Breyer
July 2012,
The American journal of pathology,
R M Breyer, and
L S Davis, and
C Nian, and
R Redha, and
B Stillman, and
H R Jacobson, and
M D Breyer
September 2006,
Pediatric research,
R M Breyer, and
L S Davis, and
C Nian, and
R Redha, and
B Stillman, and
H R Jacobson, and
M D Breyer
June 2010,
The Journal of organic chemistry,
