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Human colon sialidase has been characterized, and its activity levels in normal mucosa and colonic adenocarcinoma have been determined. Sialidase activity was maximal at pH 5.5, and was unstable with storage at 4 and -20 degrees C. The bulk of activity was pellet-associated, and could not be released with triton X-100 or 3-([3-cholamidopropyl]- dimethylammonio)-1-propanesulfonate. Using 2'-(4-methylumbelliferyl)alpha-D-N-acetylneuraminic acid as substrate, the Km and Vmax values were estimated to be 0.140 mmol/l and 63 mU/g, respectively. Furthermore, an inhibition by substrate concentrations above 1.5 mmol/l was detected. Neuraminic acid caused a competitive inhibition with a Ki of 3.5 mmol/l. A statistically significant increase (p < 0.001) in the sialidase specific activity was found in primary colonic adenocarcinoma (104.20 +/- 8.00 mU/g) compared to that of the normal mucosa (72.50 +/- 7.67 mU/g).
V S Martínez-Zorzano, and
C Feijoo, and
M Páez de la Cadena, and
M Butrón, and
A Fernández-Briera, and
F J Rodríguez-Berrocal
January 2002,
Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic],
V S Martínez-Zorzano, and
C Feijoo, and
M Páez de la Cadena, and
M Butrón, and
A Fernández-Briera, and
F J Rodríguez-Berrocal
September 1978,
Cancer research,
V S Martínez-Zorzano, and
C Feijoo, and
M Páez de la Cadena, and
M Butrón, and
A Fernández-Briera, and
F J Rodríguez-Berrocal
January 1984,
The Tohoku journal of experimental medicine,
V S Martínez-Zorzano, and
C Feijoo, and
M Páez de la Cadena, and
M Butrón, and
A Fernández-Briera, and
F J Rodríguez-Berrocal
January 1981,
Digestion,
V S Martínez-Zorzano, and
C Feijoo, and
M Páez de la Cadena, and
M Butrón, and
A Fernández-Briera, and
F J Rodríguez-Berrocal
January 1992,
Acta oncologica (Stockholm, Sweden),
V S Martínez-Zorzano, and
C Feijoo, and
M Páez de la Cadena, and
M Butrón, and
A Fernández-Briera, and
F J Rodríguez-Berrocal
February 1995,
American journal of surgery,
V S Martínez-Zorzano, and
C Feijoo, and
M Páez de la Cadena, and
M Butrón, and
A Fernández-Briera, and
F J Rodríguez-Berrocal
September 1987,
Cancer research,
V S Martínez-Zorzano, and
C Feijoo, and
M Páez de la Cadena, and
M Butrón, and
A Fernández-Briera, and
F J Rodríguez-Berrocal
July 1996,
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies,
V S Martínez-Zorzano, and
C Feijoo, and
M Páez de la Cadena, and
M Butrón, and
A Fernández-Briera, and
F J Rodríguez-Berrocal
November 2002,
Biochemistry. Biokhimiia,
V S Martínez-Zorzano, and
C Feijoo, and
M Páez de la Cadena, and
M Butrón, and
A Fernández-Briera, and
F J Rodríguez-Berrocal
January 1998,
Acta oncologica (Stockholm, Sweden),
