Effects of 60Co gamma-rays and 252Cf neutrons on human sperm chromosomes were studied using our interspecific in vitro fertilization system to estimate relative biological effectiveness (RBE) of neutrons. Semen samples were exposed to 0.5, 1.0 and 2.0 Gy of 60Co gamma-rays at 1.7 cGy/ min and 0.25, 0.5 and 1.0 Gy of 252Cf radiation at 1.3-1.7 cGy/ min. In the 60Co experiment, 509 spermatozoa from controls and 902 spermatozoa from the irradiated groups were karyotyped, while in the 252Cf experiment 460 control and 804 irradiated spermatozoa were analysed. In both 60Co and 252Cf experiments, incidences of spermatozoa with radiation-induced structural chromosome aberrations increased linearly with increase of dosage. The RBE of 252Cf neutrons for the induction of chromosomally abnormal spermatozoa was estimated to be 1.6. The number of induced structural chromosome aberrations per spermatozoon also increased linearly. The RBE of neutrons for this index was 2.0. Among structural chromosome aberrations observed, chromosome-type breaks were predominant in both 60Co and 252Cf experiments, and they showed a significant linear dose-dependent increase. Other types of aberrations such as chromosome-type exchanges and chromatid-type breaks also increased linearly with increase in dose. The RBEs of 252Cf neutrons for the induction of these three types of aberrations were 1.6, 3.2 and 3.9, respectively. Thus, the RBEs of neutrons for the induction of chromosome aberrations were smaller in human spermatozoa than in human lymphocytes, and mouse spermatogonia and embryos. This result is discussed from the point of view of DNA-repairing capacity of oocytes.