Comparison of different methods of maintenance of Malassezia pachydermatis (s. Pityrosporum pachydermatis) strains. 1996

B Dworecka-Kaszak, and F N Toka
Warsaw Agricultural University, Faculty of Veterinary Medicine, Department of Microbiology, Poland.

The maintenance of Malassezia pachydermatis isolated from canine otitis and dermatitis was compared on Sabouraud Dextrose Agar slants under liquid paraffin, an broth and in water, and by paper replica method, silica gel method, freeze-drying and deep freezing at-80 degrees C. Hydrolase production before and after storage was determined with API-ZYM Bio-Merieux kits. Deep freezing and freeze-drying were the best methods of maintaining M. pachydermatis. Hydrolase production was stable throughout the maintenance period.

UI MeSH Term Description Entries
D008290 Malassezia A mitosporic fungal genus that causes a variety of skin disorders. Malassezia furfur (Pityrosporum orbiculare) causes TINEA VERSICOLOR. Pityrosporum,Pityrosporum ovale,Saccharomyces ovalis,Malassezia furfur,Pityrosporum orbiculare
D009172 Mycology The study of the structure, growth, function, genetics, and reproduction of fungi, and MYCOSES.
D009181 Mycoses Diseases caused by FUNGI. Fungus Diseases,Fungal Diseases,Fungal Infections,Fungus Infections,Disease, Fungal,Disease, Fungus,Diseases, Fungal,Diseases, Fungus,Fungal Disease,Fungal Infection,Fungus Disease,Fungus Infection,Infection, Fungal,Infection, Fungus,Infections, Fungal,Infections, Fungus
D010032 Otitis Externa Inflammation of the OUTER EAR including the external EAR CANAL, cartilages of the auricle (EAR CARTILAGE), and the TYMPANIC MEMBRANE. External Ear Inflammation,External Otitis,Ear Inflammation, External,Externa, Otitis,External Ear Inflammations,External Otitides,Inflammation, External Ear,Otitides, External,Otitis, External
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D004283 Dog Diseases Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used. Canine Diseases,Canine Disease,Disease, Canine,Disease, Dog,Diseases, Canine,Diseases, Dog,Dog Disease
D004285 Dogs The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065) Canis familiaris,Dog
D006867 Hydrolases Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3. Hydrolase
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

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