-transparent.png){kind=logo}
The crystal structure of the xanthine oxidase-related molybdenum-iron protein aldehyde oxido-reductase from the sulfate reducing anaerobic Gram-negative bacterium Desulfovibrio gigas (Mop) was analyzed in its desulfo-, sulfo-, oxidized, reduced, and alcohol-bound forms at 1.8-A resolution. In the sulfo-form the molybdenum molybdopterin cytosine dinucleotide cofactor has a dithiolene-bound fac-[Mo, = O, = S, ---(OH2)] substructure. Bound inhibitory isopropanol in the inner compartment of the substrate binding tunnel is a model for the Michaelis complex of the reaction with aldehydes (H-C = O,-R). The reaction is proposed to proceed by transfer of the molybdenum-bound water molecule as OH- after proton transfer to Glu-869 to the carbonyl carbon of the substrate in concert with hydride transfer to the sulfido group to generate [MoIV, = O, -SH, ---(O-C = O, -R)). Dissociation of the carboxylic acid product may be facilitated by transient binding of Glu-869 to the molybdenum. The metal-bound water is replenished from a chain of internal water molecules. A second alcohol binding site in the spacious outer compartment may cause the strong substrate inhibition observed. This compartment is the putative binding site of large inhibitors of xanthine oxidase.
R Huber, and
P Hof, and
R O Duarte, and
J J Moura, and
I Moura, and
M Y Liu, and
J LeGall, and
R Hille, and
M Archer, and
M J Romão
April 2006,
Current opinion in chemical biology,
R Huber, and
P Hof, and
R O Duarte, and
J J Moura, and
I Moura, and
M Y Liu, and
J LeGall, and
R Hille, and
M Archer, and
M J Romão
January 2002,
Metal ions in biological systems,
R Huber, and
P Hof, and
R O Duarte, and
J J Moura, and
I Moura, and
M Y Liu, and
J LeGall, and
R Hille, and
M Archer, and
M J Romão
March 2015,
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry,
R Huber, and
P Hof, and
R O Duarte, and
J J Moura, and
I Moura, and
M Y Liu, and
J LeGall, and
R Hille, and
M Archer, and
M J Romão
August 1965,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
R Huber, and
P Hof, and
R O Duarte, and
J J Moura, and
I Moura, and
M Y Liu, and
J LeGall, and
R Hille, and
M Archer, and
M J Romão
May 1954,
Annals of the New York Academy of Sciences,
R Huber, and
P Hof, and
R O Duarte, and
J J Moura, and
I Moura, and
M Y Liu, and
J LeGall, and
R Hille, and
M Archer, and
M J Romão
December 2001,
The Journal of biological chemistry,
R Huber, and
P Hof, and
R O Duarte, and
J J Moura, and
I Moura, and
M Y Liu, and
J LeGall, and
R Hille, and
M Archer, and
M J Romão
October 1999,
Biochemistry,
R Huber, and
P Hof, and
R O Duarte, and
J J Moura, and
I Moura, and
M Y Liu, and
J LeGall, and
R Hille, and
M Archer, and
M J Romão
September 2000,
Proceedings of the National Academy of Sciences of the United States of America,
R Huber, and
P Hof, and
R O Duarte, and
J J Moura, and
I Moura, and
M Y Liu, and
J LeGall, and
R Hille, and
M Archer, and
M J Romão
August 1995,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
R Huber, and
P Hof, and
R O Duarte, and
J J Moura, and
I Moura, and
M Y Liu, and
J LeGall, and
R Hille, and
M Archer, and
M J Romão
January 1997,
Annual review of biochemistry,
