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The uptake characteristics of trientine by rat intestinal brush-border membrane vesicles were studied. The uptake characteristics of trientine were similar to those of the physiological polyamines with respect to the excessive accumulation in vesicles, the pH dependency, the temperature dependency and the ineffectiveness of K+ diffusion potential (inside negative). The initial uptake of trientine was saturable with a K(m) value of 1.13 mM, which was larger than that of spermine and spermidine. Furthermore, the uptake rate of trientine was dose-dependently inhibited by spermine and spermidine. Spermine competitively inhibited the uptake of trientine with a Ki value of 18.6 microM, and it was close to the K(m) value for spermine (30.4 microM). These data suggested that the uptake of trientine was similar to that of spermine and spermidine in rat small intestinal brush-border membrane vesicles, and these polyamines seem to inhibit the absorption of trientine from the gastrointestinal tract.
R Tanabe, and
M Kobayashi, and
M Sugawara, and
K Iseki, and
K Miyazaki
September 1991,
Biochimica et biophysica acta,
R Tanabe, and
M Kobayashi, and
M Sugawara, and
K Iseki, and
K Miyazaki
April 1991,
Gut,
R Tanabe, and
M Kobayashi, and
M Sugawara, and
K Iseki, and
K Miyazaki
November 1992,
Biochimica et biophysica acta,
R Tanabe, and
M Kobayashi, and
M Sugawara, and
K Iseki, and
K Miyazaki
January 1988,
Progress in clinical and biological research,
R Tanabe, and
M Kobayashi, and
M Sugawara, and
K Iseki, and
K Miyazaki
July 1992,
Biochemistry international,
R Tanabe, and
M Kobayashi, and
M Sugawara, and
K Iseki, and
K Miyazaki
May 1992,
Biochemical Society transactions,
R Tanabe, and
M Kobayashi, and
M Sugawara, and
K Iseki, and
K Miyazaki
September 1993,
The Journal of membrane biology,
R Tanabe, and
M Kobayashi, and
M Sugawara, and
K Iseki, and
K Miyazaki
December 1992,
The Journal of pharmacy and pharmacology,
R Tanabe, and
M Kobayashi, and
M Sugawara, and
K Iseki, and
K Miyazaki
January 1990,
The Journal of antimicrobial chemotherapy,
R Tanabe, and
M Kobayashi, and
M Sugawara, and
K Iseki, and
K Miyazaki
September 1992,
The Journal of pharmacy and pharmacology,
