BIOMAT Database Logo BIOMAT Database Logo

Mechanisms underlying neutrophil adhesion to apical epithelial membranes. 1996

J Meenan, and M Mevissen, and H Monajemi, and S A Radema, and H R Soule, and M Moyle, and G N Tytgat, and S J van Deventer
Department of Haemostasis, Inflammation, Atherosclerosis and Thrombosis (HIAT) Research, Academic Medical Centre, University of Amsterdam, Netherlands.

Crypt abscesses allow prolonged apposition of activated neutrophils to the epithelial surface of the colon. Adhesion of neutrophils to both the vascular endothelium and basolateral epithelial membrane share common effector molecules but are distinct processes. This study aimed to define the mechanisms that effect adhesion, independent of transmigration, to the apical epithelium. HT29 (cl 19A) cells were grown to confluency and incubated with neutrophils under conditions of: (i) neutrophil stimulation with phorbol-myristate-acetate; (ii) monolayer stimulation with interferon gamma, tumour necrosis factor alpha (IFN gamma, TNF alpha); and (iii) recent epithelial cell trypsinisation. These experiments were carried out in the presence of neutralising antibodies to CD18, CD11b, LFA-1, E-selectin, P-selectin, intracellular adhesion molecule 1 (ICAM-1), and ICAM-2; a novel CD11b/CD18 antagonist, neutrophil inhibitory factor (rNIF); adenosine receptor agonists (5'N-ethycarboxamido adenosine/N6-cylopentyladenosine (NECA/CPA)) and a platelet activating factor (PAF) receptor antagonist lexipafant. Adhesion of stimulated neutrophils to resting monolayers was Mac-1, CD18 dependent and ICAM-1, ICAM-2, E-selectin, P-selectin, PAF independent. Cytokine activated monolayers exhibited higher binding of neutrophils which was inhibited by rNIF and aCD18. Recently trypsinised monolayers bound neutrophils in a CD11b/CD18 and CD18 independent manner. Adenosine agonists failed to influence neutrophil adhesion under any condition. This study shows neutrophil adhesion to apical epithelial membranes is similar to that at the epithelial basolateral membrane, though different to that seen at the vascular endothelium. These results highlight regional differences in neutrophil adhesion molecule usage.

UI MeSH Term Description Entries
D008565 Membrane Proteins Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors. Cell Membrane Protein,Cell Membrane Proteins,Cell Surface Protein,Cell Surface Proteins,Integral Membrane Proteins,Membrane-Associated Protein,Surface Protein,Surface Proteins,Integral Membrane Protein,Membrane Protein,Membrane-Associated Proteins,Membrane Associated Protein,Membrane Associated Proteins,Membrane Protein, Cell,Membrane Protein, Integral,Membrane Proteins, Integral,Protein, Cell Membrane,Protein, Cell Surface,Protein, Integral Membrane,Protein, Membrane,Protein, Membrane-Associated,Protein, Surface,Proteins, Cell Membrane,Proteins, Cell Surface,Proteins, Integral Membrane,Proteins, Membrane,Proteins, Membrane-Associated,Proteins, Surface,Surface Protein, Cell
D009504 Neutrophils Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes. LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D002448 Cell Adhesion Adherence of cells to surfaces or to other cells. Adhesion, Cell,Adhesions, Cell,Cell Adhesions
D002999 Clone Cells A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed) Clones,Cell, Clone,Cells, Clone,Clone,Clone Cell
D003110 Colonic Neoplasms Tumors or cancer of the COLON. Cancer of Colon,Colon Adenocarcinoma,Colon Cancer,Cancer of the Colon,Colon Neoplasms,Colonic Cancer,Neoplasms, Colonic,Adenocarcinoma, Colon,Adenocarcinomas, Colon,Cancer, Colon,Cancer, Colonic,Cancers, Colon,Cancers, Colonic,Colon Adenocarcinomas,Colon Cancers,Colon Neoplasm,Colonic Cancers,Colonic Neoplasm,Neoplasm, Colon,Neoplasm, Colonic,Neoplasms, Colon
D006023 Glycoproteins Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins. C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014407 Tumor Cells, Cultured Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured
D015801 Helminth Proteins Proteins found in any species of helminth. Helminth Protein,Protein, Helminth,Proteins, Helminth
D015815 Cell Adhesion Molecules Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis. Cell Adhesion Molecule,Intercellular Adhesion Molecule,Intercellular Adhesion Molecules,Leukocyte Adhesion Molecule,Leukocyte Adhesion Molecules,Saccharide-Mediated Cell Adhesion Molecules,Saccharide Mediated Cell Adhesion Molecules,Adhesion Molecule, Cell,Adhesion Molecule, Intercellular,Adhesion Molecule, Leukocyte,Adhesion Molecules, Cell,Adhesion Molecules, Intercellular,Adhesion Molecules, Leukocyte,Molecule, Cell Adhesion,Molecule, Intercellular Adhesion,Molecule, Leukocyte Adhesion,Molecules, Cell Adhesion,Molecules, Intercellular Adhesion,Molecules, Leukocyte Adhesion

Related Publications

J Meenan, and M Mevissen, and H Monajemi, and S A Radema, and H R Soule, and M Moyle, and G N Tytgat, and S J van Deventer
Probiotic Adhesion to Skin Keratinocytes and Underlying Mechanisms.
September 2022, Biology,
J Meenan, and M Mevissen, and H Monajemi, and S A Radema, and H R Soule, and M Moyle, and G N Tytgat, and S J van Deventer
Mechanisms underlying neutrophil-mediated monocyte recruitment.
November 2009, Blood,
J Meenan, and M Mevissen, and H Monajemi, and S A Radema, and H R Soule, and M Moyle, and G N Tytgat, and S J van Deventer
Epithelial cell adhesion mechanisms.
December 1989, The Journal of membrane biology,
J Meenan, and M Mevissen, and H Monajemi, and S A Radema, and H R Soule, and M Moyle, and G N Tytgat, and S J van Deventer
Substance P increases neutrophil adhesion to bronchial epithelial cells.
February 1994, Journal of immunology (Baltimore, Md. : 1950),
J Meenan, and M Mevissen, and H Monajemi, and S A Radema, and H R Soule, and M Moyle, and G N Tytgat, and S J van Deventer
Molecular mechanisms underlying TGF-ß/Hippo signaling crosstalks - Role of baso-apical epithelial cell polarity.
May 2018, The international journal of biochemistry & cell biology,
J Meenan, and M Mevissen, and H Monajemi, and S A Radema, and H R Soule, and M Moyle, and G N Tytgat, and S J van Deventer
The molecular mechanisms underlying mussel adhesion.
November 2019, Nanoscale advances,
J Meenan, and M Mevissen, and H Monajemi, and S A Radema, and H R Soule, and M Moyle, and G N Tytgat, and S J van Deventer
Infection of human intestinal epithelial cells with invasive bacteria upregulates apical intercellular adhesion molecule-1 (ICAM)-1) expression and neutrophil adhesion.
July 1996, The Journal of clinical investigation,
J Meenan, and M Mevissen, and H Monajemi, and S A Radema, and H R Soule, and M Moyle, and G N Tytgat, and S J van Deventer
Neutrophil priming: pathophysiological consequences and underlying mechanisms.
May 1998, Clinical science (London, England : 1979),
J Meenan, and M Mevissen, and H Monajemi, and S A Radema, and H R Soule, and M Moyle, and G N Tytgat, and S J van Deventer
Trafficking to the apical and basolateral membranes in polarized epithelial cells.
July 2014, Journal of the American Society of Nephrology : JASN,
J Meenan, and M Mevissen, and H Monajemi, and S A Radema, and H R Soule, and M Moyle, and G N Tytgat, and S J van Deventer
Neural mechanisms underlying brain waves: from neural membranes to networks.
August 1991, Electroencephalography and clinical neurophysiology,
Copied contents to your clipboard!