BIOMAT Database Logo BIOMAT Database Logo

Foetal ventral mesencephalic cell suspension grafts to the 6-hydroxydopamine-lesioned rat reduce the rate of dopamine uptake in the contralateral striatum. 1996

C D Earl, and A Marburger, and D Schönfuss, and J Sautter, and S Strecker, and Z Yang, and A Kupsch, and W H Oertel, and R Morgenstern
Institute of Physiology, University of Munich, Germany. c.earl@lrz.uni-muenchen.de

The present study employed fast cyclic voltammetry, at carbon-fibre microelectrodes, to monitor and compare the rate of dopamine uptake in the rat striatum contralateral to (a) the 6-hydroxydopamine (6-OHDA)-lesioned/grafted striatum and (b) the 6-OHDA-lesioned/ sham grafted striatum. Cell suspensions of foetal rat ventral mesencephalic tissue were grafted into the dopamine-depleted striatum of unilaterally 6-OHDA-lesioned rats. Six weeks after grafting, animals with functional, mature grafts were monitored for dopamine elimination in the contralateral striatum following electrical stimulation of the median forebrain bundle, before and after treatment with the dopamine uptake inhibitor GBR 12909. Compared to animals with sham grafts, amphetamine-amplified rotational behaviour was significantly reduced in animals with grafts of foetal ventral mesencephalic tissue. Fast cyclic voltammetric measurements followed by evaluation with the aid of a kinetic model revealed that in grafted animals, the rate of dopamine uptake via the high affinity uptake mechanism, following treatment with GBR 12909, was significantly reduced when compared to sham grafted animals.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D007839 Functional Laterality Behavioral manifestations of cerebral dominance in which there is preferential use and superior functioning of either the left or the right side, as in the preferred use of the right hand or right foot. Ambidexterity,Behavioral Laterality,Handedness,Laterality of Motor Control,Mirror Writing,Laterality, Behavioral,Laterality, Functional,Mirror Writings,Motor Control Laterality,Writing, Mirror,Writings, Mirror
D008636 Mesencephalon The middle of the three primitive cerebral vesicles of the embryonic brain. Without further subdivision, midbrain develops into a short, constricted portion connecting the PONS and the DIENCEPHALON. Midbrain contains two major parts, the dorsal TECTUM MESENCEPHALI and the ventral TEGMENTUM MESENCEPHALI, housing components of auditory, visual, and other sensorimoter systems. Midbrain,Mesencephalons,Midbrains
D008839 Microelectrodes Electrodes with an extremely small tip, used in a voltage clamp or other apparatus to stimulate or record bioelectric potentials of single cells intracellularly or extracellularly. (Dorland, 28th ed) Electrodes, Miniaturized,Electrode, Miniaturized,Microelectrode,Miniaturized Electrode,Miniaturized Electrodes
D010879 Piperazines Compounds that are derived from PIPERAZINE.
D004298 Dopamine One of the catecholamine NEUROTRANSMITTERS in the brain. It is derived from TYROSINE and is the precursor to NOREPINEPHRINE and EPINEPHRINE. Dopamine is a major transmitter in the extrapyramidal system of the brain, and important in regulating movement. A family of receptors (RECEPTORS, DOPAMINE) mediate its action. Hydroxytyramine,3,4-Dihydroxyphenethylamine,4-(2-Aminoethyl)-1,2-benzenediol,Dopamine Hydrochloride,Intropin,3,4 Dihydroxyphenethylamine,Hydrochloride, Dopamine
D004558 Electric Stimulation Use of electric potential or currents to elicit biological responses. Stimulation, Electric,Electrical Stimulation,Electric Stimulations,Electrical Stimulations,Stimulation, Electrical,Stimulations, Electric,Stimulations, Electrical
D005260 Female Females
D000661 Amphetamine A powerful central nervous system stimulant and sympathomimetic. Amphetamine has multiple mechanisms of action including blocking uptake of adrenergics and dopamine, stimulation of release of monamines, and inhibiting monoamine oxidase. Amphetamine is also a drug of abuse and a psychotomimetic. The l- and the d,l-forms are included here. The l-form has less central nervous system activity but stronger cardiovascular effects. The d-form is DEXTROAMPHETAMINE. Desoxynorephedrin,Levoamphetamine,Phenopromin,l-Amphetamine,Amfetamine,Amphetamine Sulfate,Amphetamine Sulfate (2:1),Centramina,Fenamine,Mydrial,Phenamine,Thyramine,levo-Amphetamine,Sulfate, Amphetamine,l Amphetamine,levo Amphetamine
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

C D Earl, and A Marburger, and D Schönfuss, and J Sautter, and S Strecker, and Z Yang, and A Kupsch, and W H Oertel, and R Morgenstern
Glial cell line-derived neurotrophic factor improves survival of ventral mesencephalic grafts to the 6-hydroxydopamine lesioned striatum.
August 1997, Experimental brain research,
C D Earl, and A Marburger, and D Schönfuss, and J Sautter, and S Strecker, and Z Yang, and A Kupsch, and W H Oertel, and R Morgenstern
Comparison of mesencephalic free-floating tissue culture grafts and cell suspension grafts in the 6-hydroxydopamine-lesioned rat.
April 1998, Experimental brain research,
C D Earl, and A Marburger, and D Schönfuss, and J Sautter, and S Strecker, and Z Yang, and A Kupsch, and W H Oertel, and R Morgenstern
Restoration of dopamine overflow and clearance from the 6-hydroxydopamine lesioned rat striatum reinnervated by fetal mesencephalic grafts.
August 1994, The Journal of pharmacology and experimental therapeutics,
C D Earl, and A Marburger, and D Schönfuss, and J Sautter, and S Strecker, and Z Yang, and A Kupsch, and W H Oertel, and R Morgenstern
Intranigral ventral mesencephalic grafts and nigrostriatal injections of glial cell line-derived neurotrophic factor restore dopamine release in the striatum of 6-hydroxydopamine-lesioned rats.
April 1998, Experimental brain research,
C D Earl, and A Marburger, and D Schönfuss, and J Sautter, and S Strecker, and Z Yang, and A Kupsch, and W H Oertel, and R Morgenstern
Fetal mesencephalic grafts decrease the rate of dopamine uptake in the non-lesioned striatum of unilaterally 6-OHDA lesioned rats: an in vivo voltammetric study.
October 1995, Neuroscience letters,
C D Earl, and A Marburger, and D Schönfuss, and J Sautter, and S Strecker, and Z Yang, and A Kupsch, and W H Oertel, and R Morgenstern
Fetal ventral mesencephalic grafts functionally reduce the dopamine D2 receptor supersensitivity in partially dopamine reinnervated host striatum.
July 2000, Experimental neurology,
C D Earl, and A Marburger, and D Schönfuss, and J Sautter, and S Strecker, and Z Yang, and A Kupsch, and W H Oertel, and R Morgenstern
Intranigral grafts of fetal ventral mesencephalic tissue in adult 6-hydroxydopamine-lesioned rats can induce behavioral recovery.
January 1997, Cell transplantation,
C D Earl, and A Marburger, and D Schönfuss, and J Sautter, and S Strecker, and Z Yang, and A Kupsch, and W H Oertel, and R Morgenstern
GDNF triggers fiber outgrowth of fetal ventral mesencephalic grafts from nigra to striatum in 6-OHDA-lesioned rats.
November 1996, Cell and tissue research,
C D Earl, and A Marburger, and D Schönfuss, and J Sautter, and S Strecker, and Z Yang, and A Kupsch, and W H Oertel, and R Morgenstern
Transplantation of Mesencephalic Cell Suspension in Dopamine-Denervated Striatum of the Rat
April 1996, Experimental neurology,
C D Earl, and A Marburger, and D Schönfuss, and J Sautter, and S Strecker, and Z Yang, and A Kupsch, and W H Oertel, and R Morgenstern
Kidney cografts enhance fiber outgrowth from ventral mesencephalic grafts to the 6-OHDA-lesioned striatum, and improve behavioral recovery.
January 1998, Cell transplantation,
Copied contents to your clipboard!