-transparent.png){kind=logo}
The BACTEC radiometric culture method for detection of Mycobacterium paratuberculosis was evaluated on faeces from cattle on a farm in quarantine for Johne's disease. A multiplex polymerase chain reaction (PCR) based on the IS900 sequence specific for M paratuberculosis and a genus specific 16S rRNA region was developed and used to test cultures showing evidence of mycobacterial growth in the BACTEC liquid radiometric culture medium. Using the BACTEC-PCR combination, confirmation of M paratuberculosis from faeces and tissue of clinically affected animals was achieved within 2 to 4 weeks and 1 week, respectively, a substantial improvement on traditional culture and identification methods. The PCR provided rapid exclusion of M paratuberculosis when other Mycobacterium spp were grown. The radiometric culture medium proved to be very sensitive for culturing Mycobacterium spp.
D V Cousins, and
R J Evans, and
B R Francis
September 1993,
Veterinary microbiology,
D V Cousins, and
R J Evans, and
B R Francis
July 1994,
The Veterinary record,
D V Cousins, and
R J Evans, and
B R Francis
April 2001,
Molecular and cellular probes,
D V Cousins, and
R J Evans, and
B R Francis
May 2009,
Diagnostic microbiology and infectious disease,
D V Cousins, and
R J Evans, and
B R Francis
July 2001,
Australian veterinary journal,
D V Cousins, and
R J Evans, and
B R Francis
June 2007,
Journal of clinical microbiology,
D V Cousins, and
R J Evans, and
B R Francis
January 2004,
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
D V Cousins, and
R J Evans, and
B R Francis
April 1999,
Journal of clinical microbiology,
D V Cousins, and
R J Evans, and
B R Francis
April 1996,
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire,
D V Cousins, and
R J Evans, and
B R Francis
September 1997,
Veterinary microbiology,
