BIOMAT Database Logo BIOMAT Database Logo

Cyclic adenosine-3',5'-monophosphate production is greater in rabbit duodenal crypt than in villus cells. 1996

M Amelsberg, and A Amelsberg, and M A Ainsworth, and D L Hogan, and J I Isenberg
Division of Gastroenterology, Dept. of Medicine, University of California at San Diego, California, USA.

BACKGROUND Duodenal surface epithelial cells secrete bicarbonate. Agonists of duodenal alkaline secretion (such as vasoactive intestinal polypeptide (VIP), prostaglandin E2 (PGE(2)), and forskolin) increase intracellular cyclic adenosine-3', 5-monophosphate (cAMP), and cAMP stimulates Cl-HCO(3)- exchange in duodenal brush border membrane vesicles. As intestinal villus and crypt cells differ in function, our aims were to contrast cAMP generation in duodenal villus versus crypt cells in response to VIP, PGE(2), and forskolin. METHODS Villus and crypt rabbit duodenal enterocytes were isolated by calcium chelation. To prevent the degradation of cAMP in vitro, phosphodiesterase activity was inhibited. cAMP production was quantitated in response to VIP (10(-10)-10(-5)M), PGE(2) (10(-10)-10(-4)M), and forskolin (10(-8)-10(-3)M). RESULTS In crypt cells cAMP generation was approximately 10-fold greater (P < 0.001) in response to VIP, PGE(2), and forskolin than to villus cells. The relative orders of potency (that is, D(50), VIP > PGE(2) > forskolin) and efficacy (that is, V max, forskolin > VIP and PGE(2)) were similar in villus and crypt cells. CONCLUSIONS cAMP production is greater in duodenal crypt than in villus enterocytes at rest and in response to forskolin, VIP, and PGE(2), suggesting that alkaline secretion may differ along the villus-to-crypt axis.

UI MeSH Term Description Entries
D007413 Intestinal Mucosa Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI. Intestinal Epithelium,Intestinal Glands,Epithelium, Intestinal,Gland, Intestinal,Glands, Intestinal,Intestinal Gland,Mucosa, Intestinal
D008297 Male Males
D008871 Microvilli Minute projections of cell membranes which greatly increase the surface area of the cell. Brush Border,Striated Border,Border, Brush,Border, Striated,Borders, Brush,Borders, Striated,Brush Borders,Microvillus,Striated Borders
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D004386 Duodenum The shortest and widest portion of the SMALL INTESTINE adjacent to the PYLORUS of the STOMACH. It is named for having the length equal to about the width of 12 fingers. Duodenums
D005576 Colforsin Potent activator of the adenylate cyclase system and the biosynthesis of cyclic AMP. From the plant COLEUS FORSKOHLII. Has antihypertensive, positive inotropic, platelet aggregation inhibitory, and smooth muscle relaxant activities; also lowers intraocular pressure and promotes release of hormones from the pituitary gland. Coleonol,Forskolin,N,N-Dimethyl-beta-alanine-5-(acetyloxy)-3-ethenyldodecahydro-10,10b-dihydroxy-3,4a,7,7,10a-pentamethyl-1-oxo-1H-naphtho(2,1-b)pyran-6-yl Ester HCl,NKH 477,NKH-477,NKH477
D000242 Cyclic AMP An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Adenosine Cyclic 3',5'-Monophosphate,Adenosine Cyclic 3,5 Monophosphate,Adenosine Cyclic Monophosphate,Adenosine Cyclic-3',5'-Monophosphate,Cyclic AMP, (R)-Isomer,Cyclic AMP, Disodium Salt,Cyclic AMP, Monoammonium Salt,Cyclic AMP, Monopotassium Salt,Cyclic AMP, Monosodium Salt,Cyclic AMP, Sodium Salt,3',5'-Monophosphate, Adenosine Cyclic,AMP, Cyclic,Adenosine Cyclic 3',5' Monophosphate,Cyclic 3',5'-Monophosphate, Adenosine,Cyclic Monophosphate, Adenosine,Cyclic-3',5'-Monophosphate, Adenosine,Monophosphate, Adenosine Cyclic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001639 Bicarbonates Inorganic salts that contain the -HCO3 radical. They are an important factor in determining the pH of the blood and the concentration of bicarbonate ions is regulated by the kidney. Levels in the blood are an index of the alkali reserve or buffering capacity. Bicarbonate,Bicarbonate Ions,Hydrogen Carbonates,Bicarbonate Ion,Carbonic Acid Ions,Hydrogen Carbonate,Carbonate, Hydrogen,Carbonates, Hydrogen,Ion, Bicarbonate,Ions, Bicarbonate,Ions, Carbonic Acid

Related Publications

M Amelsberg, and A Amelsberg, and M A Ainsworth, and D L Hogan, and J I Isenberg
Cruising the villus-to-crypt axis: the role of cyclic 3',5'-guanosine monophosphate in duodenal bicarbonate secretion.
December 1996, Gastroenterology,
M Amelsberg, and A Amelsberg, and M A Ainsworth, and D L Hogan, and J I Isenberg
Dopamine and vasoactive intestinal peptide stimulate cyclic adenosine-3',5'-monophosphate formation in isolated rat villus and crypt duodenocytes.
September 1993, Acta physiologica Scandinavica,
M Amelsberg, and A Amelsberg, and M A Ainsworth, and D L Hogan, and J I Isenberg
[Cyclic adenosine 3',5'-monophosphate].
January 1972, Postepy biochemii,
M Amelsberg, and A Amelsberg, and M A Ainsworth, and D L Hogan, and J I Isenberg
Cyclic adenosine 3':5'-monophosphate phosphodiesterase activity in malignant and cyclic adenosine 3':5'-monophosphate-induced "differentiated" neuroblastoma cells.
January 1975, Cancer research,
M Amelsberg, and A Amelsberg, and M A Ainsworth, and D L Hogan, and J I Isenberg
Effects of adenosine 5'-monophosphate and adenosine 3',5'-cyclic monophosphate on l cells.
November 1975, Journal of cell science,
M Amelsberg, and A Amelsberg, and M A Ainsworth, and D L Hogan, and J I Isenberg
Functional pool of cyclic adenosine 3',5'-monophosphate in rabbit platelets.
February 1983, Thrombosis and haemostasis,
M Amelsberg, and A Amelsberg, and M A Ainsworth, and D L Hogan, and J I Isenberg
Analysis of cyclic 3',5'-adenosine monophosphate and cyclic 3',5'-guanosine monophosphate.
January 1971, Methods of biochemical analysis,
M Amelsberg, and A Amelsberg, and M A Ainsworth, and D L Hogan, and J I Isenberg
Elevated arteriolar adenosine 3',5'-cyclic monophosphate production by SHR.
September 1982, The American journal of physiology,
M Amelsberg, and A Amelsberg, and M A Ainsworth, and D L Hogan, and J I Isenberg
Effects of intracellularly applied cyclic 3',5'-adenosine monophosphate and dibutyryl cyclic 3',5'-adenosine monophosphate on the electrical activity of sinoatrial nodal cells of the rabbit.
July 1974, The Journal of pharmacology and experimental therapeutics,
M Amelsberg, and A Amelsberg, and M A Ainsworth, and D L Hogan, and J I Isenberg
Cyclic adenosine 3', 5'-monophosphate in mycobacteria.
December 1976, Indian journal of biochemistry & biophysics,
Copied contents to your clipboard!