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With a 3 x 3 mu m(2) proton microbeam spatial distributions of Na, Mg, P, S, K, Ca and Fe were measured via PIXE in 50 x 50 mu m(2) areas of rat heart, sliced into 10-15 mu m thick cryosections. The isolated rat hearts were subjected to normal perfusion, ischemia and reperfusion. Substantial changes in the elemental distribution were found in tissue after 40 min. of reperfusion, particularly indicated by locally elevated Ca and decreased K levels. Electron microscopic examination was used for assessment of artefacts due to sample preparation and handling. Results of stained cryosections analyzed via STIM demonstrated that this latter technique can be employed prior to PIXE analysis to localize individual cells in freeze-dried cryosections.
B A Verhoef, and
J J de Goeij, and
P H Mutsaers, and
M J de Voigt, and
P H Bomans, and
P M Frederik, and
G J van der Vusse
January 1996,
Circulation,
B A Verhoef, and
J J de Goeij, and
P H Mutsaers, and
M J de Voigt, and
P H Bomans, and
P M Frederik, and
G J van der Vusse
January 1991,
The American journal of physiology,
B A Verhoef, and
J J de Goeij, and
P H Mutsaers, and
M J de Voigt, and
P H Bomans, and
P M Frederik, and
G J van der Vusse
July 1999,
Free radical biology & medicine,
B A Verhoef, and
J J de Goeij, and
P H Mutsaers, and
M J de Voigt, and
P H Bomans, and
P M Frederik, and
G J van der Vusse
March 1992,
European journal of pharmacology,
B A Verhoef, and
J J de Goeij, and
P H Mutsaers, and
M J de Voigt, and
P H Bomans, and
P M Frederik, and
G J van der Vusse
July 1989,
The American journal of physiology,
B A Verhoef, and
J J de Goeij, and
P H Mutsaers, and
M J de Voigt, and
P H Bomans, and
P M Frederik, and
G J van der Vusse
January 1999,
Prostaglandins, leukotrienes, and essential fatty acids,
B A Verhoef, and
J J de Goeij, and
P H Mutsaers, and
M J de Voigt, and
P H Bomans, and
P M Frederik, and
G J van der Vusse
July 1999,
Circulation,
B A Verhoef, and
J J de Goeij, and
P H Mutsaers, and
M J de Voigt, and
P H Bomans, and
P M Frederik, and
G J van der Vusse
May 1993,
European journal of pharmacology,
B A Verhoef, and
J J de Goeij, and
P H Mutsaers, and
M J de Voigt, and
P H Bomans, and
P M Frederik, and
G J van der Vusse
April 1999,
Molecular and cellular biochemistry,
B A Verhoef, and
J J de Goeij, and
P H Mutsaers, and
M J de Voigt, and
P H Bomans, and
P M Frederik, and
G J van der Vusse
November 1998,
The American journal of physiology,
