Biomaterial Database

Bromodeoxyuridine inhibition of Friend leukemia cell induction. Mechanism of reversal by deoxycytidine. 1977

M D Bick

Deoxycytidine (dC) reverses bromodeoxyuridine (brdUrd) inhibition of induction in Friend leukemia cells only if added within the first 6 h after the addition of brdUrd. dC is shown to reduce the uptake of [3H]brdUrd into both soluble nucleotide pools and DNA, substantially expand the dTTP pool, and result in a lower level of brdUrd substitution in DNA. When the conversion of dC to thymidine nucleotides is prevented in BATH medium (containing brdUrd, aminopterin, thymidine, and hypoxanthine), dC no longer reverses brdUrd inhibition. These results show that dC exerts its primary effect via alterations in thymidine pools and probably through the resultant lower substitution of brdUrd in DNA.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D001973	Bromodeoxyuridine	A nucleoside that substitutes for thymidine in DNA and thus acts as an antimetabolite. It causes breaks in chromosomes and has been proposed as an antiviral and antineoplastic agent. It has been given orphan drug status for use in the treatment of primary brain tumors.	BUdR,BrdU,Bromouracil Deoxyriboside,Broxuridine,5-Bromo-2'-deoxyuridine,5-Bromodeoxyuridine,NSC-38297,5 Bromo 2' deoxyuridine,5 Bromodeoxyuridine,Deoxyriboside, Bromouracil
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D003841	Deoxycytidine	A nucleoside component of DNA composed of CYTOSINE and DEOXYRIBOSE.	Cytosine Deoxyribonucleoside,Cytosine Deoxyriboside,Deoxyribonucleoside, Cytosine,Deoxyriboside, Cytosine
D005622	Friend murine leukemia virus	A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) producing leukemia of the reticulum-cell type with massive infiltration of liver, spleen, and bone marrow. It infects DBA/2 and Swiss mice.	Friend Virus,Rowson-Parr Virus,Rowson Parr Virus,Virus, Friend,Virus, Rowson-Parr
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor
D014779	Virus Replication	The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.	Viral Replication,Replication, Viral,Replication, Virus,Replications, Viral,Replications, Virus,Viral Replications,Virus Replications