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1. Inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and inositol hexakisphosphate (InsP6) are produced in response to stimulation of cardiac alpha 1-adrenoceptors. While the role of Ins(1,4,5)P3 and Ins(1,4,5)P3 receptors is well-defined in many tissues including brain, the functional role of the putative InsP6-InsP6 receptor system in cardiac function is less clear. Using quantitative autoradiography, this study examined the characteristics and regional localization of [3H]-InsP6 binding sites in rat heart and compared the affinity of a range of inositol polyphosphates for [3H]-InsP6 and [3H]-Ins(1,4,5)P3 binding sites in heart and brain. 2. [3H]-InsP6 bound to a single, high affinity site in sections of rat heart (KD ranging from 22 +/- 1.9 nM in right atria to 35 +/- 2.6 nM in the interventricular septum, n = 7). The maximal number of binding sites (Bmax) ranged from 5.1 +/- 0.48 to 12 +/- 1.8 pmol mg-1 protein in left atrium and left ventricle, respectively. Inositol phosphates inhibited binding of [3H]-InsP6 with the order of potency: InsP6 > Ins(1,4,5)PS3 > inositol 1,3,4,5-tetrakisphosphate > or = inositol pentakisphosphate > Ins(1,4,5)P3 > > inositol mono- and bisphosphates, consistent with the labelling of an InsP6 binding site. 3. The Ins(1,4,5)P3 analogue, Ins(1,4,5)PS3, originally investigated as a putative selective radioligand for the Ins(1,4,5)P3 receptor, was a potent inhibitor of [3H]-InsP6 binding in all heart regions (K1 = 170-260 nM). The K1 of Ins(1,4,5)PS3 for the inhibition of [3H]-Ins(1,4,5)P3 binding in rat brain (60-220 nM) was similar to that observed for the inhibition of [3H]-InsP6 binding in heart, suggesting that Ins(1,4,5)PS3 is not a specific ligand for either Ins(1,4,5)P3 or InsP6 receptor binding sites. 4. Previous studies have detected [3H]-InsP6 binding in mitochondrial and sarcoplasmic reticulum fractions of heart and links between InsP6 and cardiac mitochondrial Ca2+ regulation have been proposed, suggesting further studies are warranted to determine the functional role(s) of InsP6 and InsP6 receptor binding sites in cardiac tissue.
K G Rowley, and
A L Gundlach, and
M Cincotta, and
W J Louis
January 1994,
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K G Rowley, and
A L Gundlach, and
M Cincotta, and
W J Louis
April 1991,
Proceedings of the National Academy of Sciences of the United States of America,
K G Rowley, and
A L Gundlach, and
M Cincotta, and
W J Louis
January 2001,
Hippocampus,
K G Rowley, and
A L Gundlach, and
M Cincotta, and
W J Louis
September 1995,
Current eye research,
K G Rowley, and
A L Gundlach, and
M Cincotta, and
W J Louis
January 1993,
The Journal of biological chemistry,
K G Rowley, and
A L Gundlach, and
M Cincotta, and
W J Louis
April 1992,
European journal of pharmacology,
K G Rowley, and
A L Gundlach, and
M Cincotta, and
W J Louis
March 1990,
Biochemical and biophysical research communications,
K G Rowley, and
A L Gundlach, and
M Cincotta, and
W J Louis
June 1990,
The Journal of biological chemistry,
K G Rowley, and
A L Gundlach, and
M Cincotta, and
W J Louis
May 1992,
The Journal of biological chemistry,
K G Rowley, and
A L Gundlach, and
M Cincotta, and
W J Louis
June 1990,
Biochemical Society transactions,
