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Quantitative regimen for the entrapping of creatine phosphokinase (CPK) under different experimental conditions was investigated for the preparation of liposomes with a high internal protein content. As revealed, introduction of 20% of positively-charged lipid (stearyl amine) into the lipid mixture and subsequent one-minute sonication resulted in maximal levels of latent enzyme activity. Maximal entrapment (6.1%) was yielded when 300 mM glucose solution (pH 7.0) AND 0.5 MG OF PROTEIN PER ML WERE USED. Liposome preparations when washed twice at 205 000 g with physiological saline showed minimal external enzyme activity. During storage of liposomes for 7 days under argon protection the internal protein content has not diminished, nor the external activity has increased.
E F Davidenkova, and
O A Rozenberg, and
E I Shvarts
February 1969,
Clinical chemistry,
E F Davidenkova, and
O A Rozenberg, and
E I Shvarts
February 1969,
Clinical chemistry,
E F Davidenkova, and
O A Rozenberg, and
E I Shvarts
September 1971,
The New England journal of medicine,
E F Davidenkova, and
O A Rozenberg, and
E I Shvarts
September 1963,
Lancet (London, England),
E F Davidenkova, and
O A Rozenberg, and
E I Shvarts
November 1976,
Archives of internal medicine,
E F Davidenkova, and
O A Rozenberg, and
E I Shvarts
May 1956,
Canadian journal of biochemistry and physiology,
E F Davidenkova, and
O A Rozenberg, and
E I Shvarts
December 1973,
Archives of general psychiatry,
E F Davidenkova, and
O A Rozenberg, and
E I Shvarts
May 1973,
The British journal of psychiatry : the journal of mental science,
E F Davidenkova, and
O A Rozenberg, and
E I Shvarts
August 1977,
The American review of respiratory disease,
E F Davidenkova, and
O A Rozenberg, and
E I Shvarts
August 1972,
British medical journal,
