BIOMAT Database Logo BIOMAT Database Logo

Inhibition of rabies virus infection by an oligodeoxynucleotide complementary to rabies virus genomic RNA. 1996

Z F Fu, and E Wickstrom, and M Jiang, and S Corisdeo, and J Yang, and B Dietzschold, and H Koprowski
Department of Microbiology and Immunology, Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107, USA.

To develop antirabies virus-specific agents, eight oligodeoxynucleotides (ODN) complementary to either rabies virus genomic RNA (negative polarity) or rabies virus transcripts (mRNA) were synthesized and tested for their activity to inhibit rabies virus infection in cell cultures. It was found that the ODN RH+1 complementary to rabies virus genomic RNA blocked almost completely rabies virus infection at concentrations as low as 2 microM, whereas ODN complementary to viral transcripts did poorly even at concentrations as high as 20 microM. The antigenomic ODN also has the ability to inhibit cell-to-cell spread of rabies virus, which is an indicator for protection of rabies virus infection in vivo. These results indicate that ODN complementary to rabies virus genomic RNA have strong ability to inhibit rabies virus infection in cell culture and may have the potential to be used for therapy in clinical rabies.

UI MeSH Term Description Entries
D011818 Rabies Acute VIRAL CNS INFECTION affecting mammals, including humans. It is caused by RABIES VIRUS and usually spread by contamination with virus-laden saliva of bites inflicted by rabid animals. Important animal vectors include the dog, cat, bat, fox, raccoon, skunk, and wolf. Encephalitic Rabies,Furious Rabies,Hydrophobia,Paralytic Rabies,Lyssa,Furious Raby,Lyssas,Rabies, Encephalitic,Rabies, Furious,Raby, Furious
D011820 Rabies virus The type species of LYSSAVIRUS causing rabies in humans and other animals. Transmission is mostly by animal bites through saliva. The virus is neurotropic multiplying in neurons and myotubes of vertebrates. Rabies viruses
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000998 Antiviral Agents Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly. Antiviral,Antiviral Agent,Antiviral Drug,Antivirals,Antiviral Drugs,Agent, Antiviral,Agents, Antiviral,Drug, Antiviral,Drugs, Antiviral
D012367 RNA, Viral Ribonucleic acid that makes up the genetic material of viruses. Viral RNA
D016376 Oligonucleotides, Antisense Short fragments of DNA or RNA that are used to alter the function of target RNAs or DNAs to which they hybridize. Anti-Sense Oligonucleotide,Antisense Oligonucleotide,Antisense Oligonucleotides,Anti-Sense Oligonucleotides,Anti Sense Oligonucleotide,Anti Sense Oligonucleotides,Oligonucleotide, Anti-Sense,Oligonucleotide, Antisense,Oligonucleotides, Anti-Sense
D016679 Genome, Viral The complete genetic complement contained in a DNA or RNA molecule in a virus. Viral Genome,Genomes, Viral,Viral Genomes
D018075 RNA, Complementary Synthetic transcripts of a specific DNA molecule or fragment, made by an in vitro transcription system. This cRNA can be labeled with radioactive uracil and then used as a probe. (King & Stansfield, A Dictionary of Genetics, 4th ed) Complementary RNA,cRNA,cRNA Probes,Probes, cRNA

Related Publications

Z F Fu, and E Wickstrom, and M Jiang, and S Corisdeo, and J Yang, and B Dietzschold, and H Koprowski
Complementary oligodeoxynucleotide mediated inhibition of tobacco mosaic virus RNA translation in vitro.
May 1988, Nucleic acids research,
Z F Fu, and E Wickstrom, and M Jiang, and S Corisdeo, and J Yang, and B Dietzschold, and H Koprowski
Inhibition of proopiomelanocortin expression by an oligodeoxynucleotide complementary to beta-endorphin mRNA.
August 1994, Proceedings of the National Academy of Sciences of the United States of America,
Z F Fu, and E Wickstrom, and M Jiang, and S Corisdeo, and J Yang, and B Dietzschold, and H Koprowski
Inhibition of mouse hepatitis virus multiplication by an oligonucleotide complementary to the leader RNA.
June 1992, The Journal of veterinary medical science,
Z F Fu, and E Wickstrom, and M Jiang, and S Corisdeo, and J Yang, and B Dietzschold, and H Koprowski
Rescue of synthetic genomic RNA analogs of rabies virus by plasmid-encoded proteins.
February 1994, Journal of virology,
Z F Fu, and E Wickstrom, and M Jiang, and S Corisdeo, and J Yang, and B Dietzschold, and H Koprowski
Rabies virus infection: an update.
April 2003, Journal of neurovirology,
Z F Fu, and E Wickstrom, and M Jiang, and S Corisdeo, and J Yang, and B Dietzschold, and H Koprowski
Rabies Virus Infection Induces Microtubule Depolymerization to Facilitate Viral RNA Synthesis by Upregulating HDAC6.
January 2017, Frontiers in cellular and infection microbiology,
Z F Fu, and E Wickstrom, and M Jiang, and S Corisdeo, and J Yang, and B Dietzschold, and H Koprowski
In vitro and in vivo inhibition of rabies virus replication by RNA interference.
January 2013, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology],
Z F Fu, and E Wickstrom, and M Jiang, and S Corisdeo, and J Yang, and B Dietzschold, and H Koprowski
[Analysis of ribonucleoproteins of influenza virus containing genomic and complementary RNA].
September 1985, Molekuliarnaia genetika, mikrobiologiia i virusologiia,
Z F Fu, and E Wickstrom, and M Jiang, and S Corisdeo, and J Yang, and B Dietzschold, and H Koprowski
Inhibition of mouse hepatitis virus multiplication by phosphorothioate analogues of oligodeoxynucleotides complementary to the leader RNA.
December 1995, The Journal of veterinary medical science,
Z F Fu, and E Wickstrom, and M Jiang, and S Corisdeo, and J Yang, and B Dietzschold, and H Koprowski
[Inhibition of hepatitis C virus by antisense oligodeoxynucleotide in vitro].
August 1997, Zhonghua yi xue za zhi,
Copied contents to your clipboard!