-transparent.png){kind=logo}
OBJECTIVE To assess effects of the inflammatory cytokines (IL-1-beta, TNF-alpha, TGF-beta 1) and dialysate effluent on synthesis of hyaluronic acid by human peritoneal mesothelial cells (HMC) in in vitro culture. METHODS Dialysate effluent was collected after the overnight dwell of Dianeal 1.5% from patients during CAPD training. HMC were obtained from omentum from nonuremic donors or were harvested from the dialysate effluent from CAPD patients. Synthesis of hyaluronic acid was studied on monolayers of HMC, which were deprived of serum 48 hours prior to experiment. Effects of cytokines were tested in a medium with low serum concentration (0.1%) or in medium mixed (1:1 v/v) with the autologous dialysate. Hyaluronic acid level in medium was measured with radioimmunoassay. RESULTS Cytokines enhanced synthesis of hyaluronic acid by HMC, and the strongest effect was induced by IL-1. Effluent dialysate stimulates synthesis of hyaluronic acid stronger than 10% FCS. Effluent dialysate and IL-1 synergistically enhance synthesis of hyaluronic acid by HMC. CONCLUSIONS Effluent dialysate from CAPD patients stimulates production of hyaluronic acid by HMC and acts synergistically with cytokines.
A Breborowicz, and
K Korybalska, and
A Grzybowski, and
K Wieczorowska-Tobis, and
L Martis, and
D G Oreopoulos
January 1994,
Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis,
A Breborowicz, and
K Korybalska, and
A Grzybowski, and
K Wieczorowska-Tobis, and
L Martis, and
D G Oreopoulos
January 2000,
Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis,
A Breborowicz, and
K Korybalska, and
A Grzybowski, and
K Wieczorowska-Tobis, and
L Martis, and
D G Oreopoulos
January 2004,
American journal of nephrology,
A Breborowicz, and
K Korybalska, and
A Grzybowski, and
K Wieczorowska-Tobis, and
L Martis, and
D G Oreopoulos
September 1994,
Kidney international,
A Breborowicz, and
K Korybalska, and
A Grzybowski, and
K Wieczorowska-Tobis, and
L Martis, and
D G Oreopoulos
January 2009,
American journal of nephrology,
A Breborowicz, and
K Korybalska, and
A Grzybowski, and
K Wieczorowska-Tobis, and
L Martis, and
D G Oreopoulos
January 1994,
Advances in peritoneal dialysis. Conference on Peritoneal Dialysis,
A Breborowicz, and
K Korybalska, and
A Grzybowski, and
K Wieczorowska-Tobis, and
L Martis, and
D G Oreopoulos
July 1995,
Kidney international,
A Breborowicz, and
K Korybalska, and
A Grzybowski, and
K Wieczorowska-Tobis, and
L Martis, and
D G Oreopoulos
November 2004,
ANZ journal of surgery,
A Breborowicz, and
K Korybalska, and
A Grzybowski, and
K Wieczorowska-Tobis, and
L Martis, and
D G Oreopoulos
January 2022,
Frontiers in medicine,
A Breborowicz, and
K Korybalska, and
A Grzybowski, and
K Wieczorowska-Tobis, and
L Martis, and
D G Oreopoulos
February 1988,
Biochemistry international,
