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Iodide inhibits several thyroid parameters through an organic intermediate, and this process has been related to thyroid autoregulation. The aim of this study was to determine the effect of iodine on thyroglobulin (Tg) synthesis in the rat thyroid cell line FRTL-5. TSH stimulated amino acid incorporation into the cells by 400% and iodine had no effect on this parameter. No effect of TSH or iodide on [35S] methionine incorporation into protein was found under our experimental conditions (approximately 80% of total [35S]methionine incorporated was found in TCA-precipitable material). TSH caused an increase in Tg synthesis, after 1 h, while iodide partially blocked the effect of TSH (control 6.4% of TCA precipitable radioactivity; TSH 10.7%; iodide 8.4%). After 24 h, the protein released into the medium was measured. TSH stimulated total protein liberation and iodide inhibited this parameter. TSH stimulated total RNA content, and iodide caused an inhibition. Northern analysis did not show inhibition by iodide of TSH-stimulated Tg mRNA levels. The present results show an inhibitory effect of excess iodide on TSH-stimulated thyroglobulin biosynthesis in FRTL-5 cells.
L Pregliasco, and
L Bocanera, and
L Krawiec, and
D Silberschmidt, and
M Pisarev, and
G Juvenal
March 2011,
Toxicology and industrial health,
L Pregliasco, and
L Bocanera, and
L Krawiec, and
D Silberschmidt, and
M Pisarev, and
G Juvenal
January 2010,
Wei sheng yan jiu = Journal of hygiene research,
L Pregliasco, and
L Bocanera, and
L Krawiec, and
D Silberschmidt, and
M Pisarev, and
G Juvenal
November 1992,
Acta endocrinologica,
L Pregliasco, and
L Bocanera, and
L Krawiec, and
D Silberschmidt, and
M Pisarev, and
G Juvenal
June 1991,
Endocrinology,
L Pregliasco, and
L Bocanera, and
L Krawiec, and
D Silberschmidt, and
M Pisarev, and
G Juvenal
February 2001,
European journal of endocrinology,
L Pregliasco, and
L Bocanera, and
L Krawiec, and
D Silberschmidt, and
M Pisarev, and
G Juvenal
December 1984,
Endocrinology,
L Pregliasco, and
L Bocanera, and
L Krawiec, and
D Silberschmidt, and
M Pisarev, and
G Juvenal
June 1986,
Endocrinology,
L Pregliasco, and
L Bocanera, and
L Krawiec, and
D Silberschmidt, and
M Pisarev, and
G Juvenal
May 2005,
Thyroid : official journal of the American Thyroid Association,
L Pregliasco, and
L Bocanera, and
L Krawiec, and
D Silberschmidt, and
M Pisarev, and
G Juvenal
April 1984,
Endocrinology,
L Pregliasco, and
L Bocanera, and
L Krawiec, and
D Silberschmidt, and
M Pisarev, and
G Juvenal
December 1991,
Molecular and cellular endocrinology,
