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Hexosaminidases (EC 3.2.1.30) A and B from human kidney cortex were purified to homogeneity by using concanavalin A affinity chromatography, ion-exchange chromatography and gel filtration. The yield of homogeneous isoenzymes improved approx. 20-fold, giving preparations of hexosaminidases A and B with specific activities of about 200 and 325 units/mg of protein respectively. The kinetic and structural properties of kidney hexosaminidase isoenzymes were studied and compared with the hexosaminidase isoenzymes from human placenta. The amino acid composition of hexosaminidase A was significantly different from that of hexosaminidase B. In the event of success in developing enzyme-replacement therapy for Tay-Sachs and Sandhoff's diseases, this modified procedure can furnish larger amounts of homogeneous isoenzymes.
J E Wiktorowicz, and
Y C Awasthi, and
A Kurosky, and
S K Srivastava
April 1977,
The Biochemical journal,
J E Wiktorowicz, and
Y C Awasthi, and
A Kurosky, and
S K Srivastava
December 1976,
The Biochemical journal,
J E Wiktorowicz, and
Y C Awasthi, and
A Kurosky, and
S K Srivastava
September 1974,
FEBS letters,
J E Wiktorowicz, and
Y C Awasthi, and
A Kurosky, and
S K Srivastava
December 1978,
Biochemical medicine,
J E Wiktorowicz, and
Y C Awasthi, and
A Kurosky, and
S K Srivastava
July 1977,
The Biochemical journal,
J E Wiktorowicz, and
Y C Awasthi, and
A Kurosky, and
S K Srivastava
November 1975,
Israel journal of medical sciences,
J E Wiktorowicz, and
Y C Awasthi, and
A Kurosky, and
S K Srivastava
January 1978,
Methods in enzymology,
J E Wiktorowicz, and
Y C Awasthi, and
A Kurosky, and
S K Srivastava
December 1970,
Biochimica et biophysica acta,
J E Wiktorowicz, and
Y C Awasthi, and
A Kurosky, and
S K Srivastava
September 1970,
The Biochemical journal,
J E Wiktorowicz, and
Y C Awasthi, and
A Kurosky, and
S K Srivastava
July 1979,
Clinica chimica acta; international journal of clinical chemistry,
