Biomaterial Database

[Esterase spectrum in the hepatocytes and Kupffer cells of regenerating liver]. 1977

A Iu Grishanova, and V I Shcherbakov, and D N Maianskiĭ

The esterase spectra of hepatocytes and Kupffer's cells from the regenerating rat liver were studied following partial hepatectomy. The starch-gen electrophoresis method was used. The relative number of Kupffer's cells, per cent of phagocytising Kupffer's cells, as well as mitotic rate of hepatocytes were determined. Hepatocyte esterase spectra from the intact liver consisted of six, whereas that of Kupffer's cells--of five zones of the enzyme activity. A "simplification" of hepatocyte esterase spectra could be seen 2 1/2 hours after partial hepatectomy. Additional activity bands appeared in 9 hours; these could be identified for a period of up to 24 hours after the operation. The initiar esterase spectrum was regained within 48 hours. The same tendency was observed in Kupffer's cells, but no full restoration of the enzyme spectra was observed 72 hours after partial hepatectomy.

UI	MeSH Term	Description	Entries
D007728	Kupffer Cells	Specialized phagocytic cells of the MONONUCLEAR PHAGOCYTE SYSTEM found on the luminal surface of the hepatic sinusoids. They filter bacteria and small foreign proteins out of the blood and dispose of worn out red blood cells.	Kupffer Cell,Cell, Kupffer,Cells, Kupffer
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008115	Liver Regeneration	Repair or renewal of hepatic tissue.	Liver Regenerations,Regeneration, Liver,Regenerations, Liver
D008297	Male		Males
D008938	Mitosis	A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.	M Phase, Mitotic,Mitotic M Phase,M Phases, Mitotic,Mitoses,Mitotic M Phases,Phase, Mitotic M,Phases, Mitotic M
D010586	Phagocytes	Cells that can carry out the process of PHAGOCYTOSIS.	Phagocyte,Phagocytic Cell,Phagocytic Cells,Cell, Phagocytic,Cells, Phagocytic
D002454	Cell Differentiation	Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.	Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D004591	Electrophoresis, Polyacrylamide Gel	Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.	Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004950	Esterases	Any member of the class of enzymes that catalyze the cleavage of an ester bond and result in the addition of water to the resulting molecules.	Esterase
D006498	Hepatectomy	Excision of all or part of the liver. (Dorland, 28th ed)	Hepatectomies