Biomaterial Database

UVB radiation suppresses the TNF-alpha-induced expression of E-selectin and ICAM-1 on cultured human umbilical vein endothelial cells. 1996

M Yamawaki, and S Futamura, and T Horio

Department of Dermatology, Kansai Medical University, Osaka, Japan.

Endothelial cells, which are involved in the development of inflammatory and immune responses, can express various kinds of cell adhesion molecules (CAM) including E-selectin and intercellular adhesion molecules-I (ICAM-I). These cell adhesion molecules and their ligands on leukocytes play an essential role in the control of extravasation of inflammatory cells. Ultraviolet B (UVB) radiation can reach the upper dermis and modulate CAM expressions on vascular endothelial cells (EC). We examined the direct effect of UVB on E-selectin and ICAM-I expression on cultured human umbilical vein endothelial cells (HUVEC) and also examined its effect on these cells induced by tumour necrosis factor-alpha (TNF-alpha), which is a potent CAM-inducer and is released by UVB radiation on the skin. Various doses of UVB were exposed to human umbilical vein endothelial cells (HUVEC) and these expressions were examined by flow cytometric analysis using FACScan; 5, 10 and 25 mJ/cm2 UVB induced neither E-selectin nor ICAM-I expression. Irradiation of HUVEC with UVB 30 min after treatment with TNF-alpha inhibited these expressions. Although the inhibition of E-selectin was observed until 12 h in a dose-dependent manner, ICAM-I expression was almost completely inhibited, even at 5 mJ/cm2 UVB. UVB irradiation before TNF-alpha stimulation showed similar effects to those obtained post-irradiation. This study has demonstrated that UVB can directly down-regulate EC functions, and the results may have implications in action mechanisms of UVB therapy.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D004730	Endothelium, Vascular	Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.	Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012867	Skin	The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
D014409	Tumor Necrosis Factor-alpha	Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.	Cachectin,TNF-alpha,Tumor Necrosis Factor Ligand Superfamily Member 2,Cachectin-Tumor Necrosis Factor,TNF Superfamily, Member 2,TNFalpha,Tumor Necrosis Factor,Cachectin Tumor Necrosis Factor,Tumor Necrosis Factor alpha
D014466	Ultraviolet Rays	That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.	Actinic Rays,Black Light, Ultraviolet,UV Light,UV Radiation,Ultra-Violet Rays,Ultraviolet Light,Ultraviolet Radiation,Actinic Ray,Light, UV,Light, Ultraviolet,Radiation, UV,Radiation, Ultraviolet,Ray, Actinic,Ray, Ultra-Violet,Ray, Ultraviolet,Ultra Violet Rays,Ultra-Violet Ray,Ultraviolet Black Light,Ultraviolet Black Lights,Ultraviolet Radiations,Ultraviolet Ray
D015536	Down-Regulation	A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.	Receptor Down-Regulation,Down-Regulation (Physiology),Downregulation,Down Regulation,Down-Regulation, Receptor
D018799	Intercellular Adhesion Molecule-1	A cell-surface ligand involved in leukocyte adhesion and inflammation. Its production is induced by gamma-interferon and it is required for neutrophil migration into inflamed tissue.	Antigens, CD54,CD54 Antigens,ICAM-1,CD54 Antigen,Antigen, CD54,Intercellular Adhesion Molecule 1
D019040	E-Selectin	Cell adhesion molecule and CD antigen that mediates neutrophil, monocyte, and memory T-cell adhesion to cytokine-activated endothelial cells. E-selectin recognizes sialylated carbohydrate groups related to the Lewis X or Lewis A family.	Antigens, CD62E,CD62E Antigens,E Selectin,ELAM-1,Endothelial Leukocyte Adhesion Molecule-1,LECAM-2,CD62E Antigen,Endothelial Leukocyte Adhesion Molecule 1,Antigen, CD62E,Selectin, E