Biomaterial Database

Isolation and identification of bacteria from activated sludge purifying petroleum wastewaters. 1995

E Bieszkiewicz, and H Boszczyk-Maleszak, and B Kaczorowska, and R Mycielski

Institute of Microbiology, University of Warsaw, Poland.

Several strains growing well in minimal media with 500 and 1000 mg/l of oil or phenol as a sole carbon source were isolated from activated sludge purifying petroleum waste waters and identified. Five of the best growing strains classified as Arthrobacter, Pseudomonas, Xanthomonas and Enterobacter were selected and their capacity to remove petroleum components and phenol (in the oil fraction of petrochemical waste waters) was studied. The enzymatic activity of the strains, including respiration intensity and dehydrogenase activity was also determined. All the examined strains were found to use oils as the sole source of carbon (percent age of the oils reduction during cultivation of the individual strains ranged from 58 to 78). Phenol was completely reduced by only one strain. The rest of the strain removed only from 7 to 24% of this compound. The activity of dehydrogenases and the respiration intensity in the presence of the studied substrates -- oil and phenol was low for all the examined strains.

UI	MeSH Term	Description	Entries
D010088	Oxidoreductases	The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)	Dehydrogenases,Oxidases,Oxidoreductase,Reductases,Dehydrogenase,Oxidase,Reductase
D010578	Petroleum	Naturally occurring complex liquid hydrocarbons which, after distillation, yield combustible fuels, petrochemicals, and lubricants.	Crude Oil,Crude Oils,Oil, Crude,Oils, Crude,Petroleums
D010636	Phenols	Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.
D001419	Bacteria	One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.	Eubacteria
D001673	Biodegradation, Environmental	Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.	Bioremediation,Phytoremediation,Natural Attenuation, Pollution,Environmental Biodegradation,Pollution Natural Attenuation
D012722	Sewage	Refuse liquid or waste matter carried off by sewers.	Sludge,Sludge Flocs
D019800	Phenol	An antiseptic and disinfectant aromatic alcohol.	Carbol,Carbolic Acid,Hydroxybenzene,Phenol, Sodium Salt,Phenolate Sodium,Sodium Phenolate,Phenolate, Sodium