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In the developing chick embryo, cranial neural crest cells, that will subsequently give rise to facial mesenchyme tissues, migrate beneath the surface ectoderm in a cell-free and hyaluronate-rich matrix. To determine how the crest cells could contribute to this matrix, we cultured crest cells from stage 9 embryos for 2 days and then labeled them for 18--20 h with various precursors of glycosaminoglycans (GAG) and glycoproteins. [3H]fucose and [3H]glucosamine were incorporated into Pronase-sensitive macromolecules associated with the cell layer, but little labeled glycoprotein was released into the medium. Hyaluronate was the major GAG synthesized and was distributed between the cells and medium. Less chondroitin-sulfate was synthesized. In comparison, older cultures as well as fibroblasts produced different proportions of GAG. Our results confirm the autoradiographic findings of Pratt et al. (1975) and suggest that crest cells may contribute GAG to the matrix during migration.
J H Greenberg, and
R M Pratt
October 1990,
Development, growth & differentiation,
J H Greenberg, and
R M Pratt
June 2010,
Proceedings of the National Academy of Sciences of the United States of America,
J H Greenberg, and
R M Pratt
April 1996,
Diabetologia,
J H Greenberg, and
R M Pratt
February 1990,
Developmental biology,
J H Greenberg, and
R M Pratt
March 2018,
Cold Spring Harbor protocols,
J H Greenberg, and
R M Pratt
March 2018,
Cold Spring Harbor protocols,
J H Greenberg, and
R M Pratt
January 1981,
Advances in neurology,
J H Greenberg, and
R M Pratt
October 2003,
Development (Cambridge, England),
J H Greenberg, and
R M Pratt
January 1996,
Biochemical Society symposium,
J H Greenberg, and
R M Pratt
August 1981,
The Biochemical journal,
